BMRB Entry 25333
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25333
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Title: BILE SALT RECOGNITION BY HUMAN LIVER FATTY ACID BINDING PROTEIN PubMed: 25639618
Deposition date: 2014-11-12 Original release date: 2015-08-25
Authors: Favretto, Filippo; Santambrogio, Carlo; D'Onofrio, Mariapina; Molinari, Henriette; Grandori, Rita; Assfalg, Michael
Citation: Favretto, Filippo; Santambrogio, Carlo; D'Onofrio, Mariapina; Molinari, Henriette; Grandori, Rita; Assfalg, Michael. "Bile salt recognition by human liver fatty acid binding protein" FEBS J. 282, 1271-1288 (2015).
Assembly members:
hL-FABP, polymer, 135 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
hL-FABP: MRGSMSFSGKYQLQSQENFE
AFMKAIGLPEELIQKGKDIK
GVSEIVQNGKHFKFTITAGS
KVIQNEFTVGEECELETMTG
EKVKTVVQLEGDNKLVTAFK
NIKSVTELNGDIITNTMTLG
DIVFKRISKRILVPR
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 250 |
15N chemical shifts | 130 |
1H chemical shifts | 130 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | human liver fatty acid binding protein | 1 |
Entities:
Entity 1, human liver fatty acid binding protein 135 residues - Formula weight is not available
First and last four residues are added for cloning and tag purposes. Variant T94A
1 | MET | ARG | GLY | SER | MET | SER | PHE | SER | GLY | LYS | ||||
2 | TYR | GLN | LEU | GLN | SER | GLN | GLU | ASN | PHE | GLU | ||||
3 | ALA | PHE | MET | LYS | ALA | ILE | GLY | LEU | PRO | GLU | ||||
4 | GLU | LEU | ILE | GLN | LYS | GLY | LYS | ASP | ILE | LYS | ||||
5 | GLY | VAL | SER | GLU | ILE | VAL | GLN | ASN | GLY | LYS | ||||
6 | HIS | PHE | LYS | PHE | THR | ILE | THR | ALA | GLY | SER | ||||
7 | LYS | VAL | ILE | GLN | ASN | GLU | PHE | THR | VAL | GLY | ||||
8 | GLU | GLU | CYS | GLU | LEU | GLU | THR | MET | THR | GLY | ||||
9 | GLU | LYS | VAL | LYS | THR | VAL | VAL | GLN | LEU | GLU | ||||
10 | GLY | ASP | ASN | LYS | LEU | VAL | THR | ALA | PHE | LYS | ||||
11 | ASN | ILE | LYS | SER | VAL | THR | GLU | LEU | ASN | GLY | ||||
12 | ASP | ILE | ILE | THR | ASN | THR | MET | THR | LEU | GLY | ||||
13 | ASP | ILE | VAL | PHE | LYS | ARG | ILE | SER | LYS | ARG | ||||
14 | ILE | LEU | VAL | PRO | ARG |
Samples:
sample_1: human liver fatty acid binding protein, [U-100% 13C; U-100% 15N], 0.5 mM; TAUROCHOLIC ACID, natural abundant, 7.5 mM; sodium phosphate, natural abundant, 10 mM; H2O, natural abundant, 93%; D2O, [U-2H], 7%
sample_conditions_1: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
BMRB | 17302 17303 19160 19188 19189 |
PDB | |
DBJ | BAI46102 |
EMBL | CAG46887 |
GB | AAA52418 AAA52419 AAH22287 AAH32801 AAX37108 |
REF | NP_001434 XP_001140263 XP_003268834 XP_003805902 XP_004029642 |
SP | P07148 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
or all simulated shifts