BMRB Entry 4079
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4079
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Title: Structure of the A-Domain of HMG1 and Its Interaction with DNA As Studied by Heteronuclear Three- and Four-Dimensional NMR Spectroscopy
Deposition date: 1997-12-07 Original release date: 2001-02-17
Authors: Hardman, Colin; Broadhurst, R.; Raine, Andrew; Grasser, Klaus; Thomas, Jean; Laue, Ernest
Citation: Hardman, Colin; Broadhurst, R.; Raine, Andrew; Grasser, Klaus; Thomas, Jean; Laue, Ernest. "Structure of the A-Domain of HMG1 and Its Interaction with DNA As Studied by Heteronuclear Three- and Four-Dimensional NMR Spectroscopy" Biochemistry 34, 16596-16607 (1995).
Assembly members:
A-domain of HMG1, polymer, 83 residues, Formula weight is not available
Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
A-domain of HMG1: GKGDPKKPRGKMSSYAFFVQ
TSREEHKKKHPDASVNFSEF
SKKCSERWKTMSAKEKGKFE
DMAKADKARYEREMKTYIPP
KGE
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 305 |
15N chemical shifts | 79 |
1H chemical shifts | 581 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | A-domain HMG1 | 1 |
Entities:
Entity 1, A-domain HMG1 83 residues - Formula weight is not available
1 | GLY | LYS | GLY | ASP | PRO | LYS | LYS | PRO | ARG | GLY | ||||
2 | LYS | MET | SER | SER | TYR | ALA | PHE | PHE | VAL | GLN | ||||
3 | THR | SER | ARG | GLU | GLU | HIS | LYS | LYS | LYS | HIS | ||||
4 | PRO | ASP | ALA | SER | VAL | ASN | PHE | SER | GLU | PHE | ||||
5 | SER | LYS | LYS | CYS | SER | GLU | ARG | TRP | LYS | THR | ||||
6 | MET | SER | ALA | LYS | GLU | LYS | GLY | LYS | PHE | GLU | ||||
7 | ASP | MET | ALA | LYS | ALA | ASP | LYS | ALA | ARG | TYR | ||||
8 | GLU | ARG | GLU | MET | LYS | THR | TYR | ILE | PRO | PRO | ||||
9 | LYS | GLY | GLU |
Samples:
sample_one: A-domain of HMG12 5 mM; D2O 10%; H2O 90%; sodium phosphate 10 mM; NaCl 0.15 M; DTT 50 uM; NaN3 .02%
sample_two: A-domain of HMG1, [U-15N], 2 5 mM; D2O 10%; H2O 90%; sodium phosphate 10 mM; NaCl 0.15 M; DTT 50 uM; NaN3 .02%
sample_three: A-domain of HMG1, [U-13C], 2 5 mM; D2O 10%; H2O 90%; sodium phosphate 10 mM; NaCl 0.15 M; DTT 50 uM; NaN3 .02%
sample_four: A-domain of HMG1, [U-15N;U-13C], 2 5 mM; D2O 10%; H2O 90%; sodium phosphate 10 mM; NaCl 0.15 M; DTT 50 uM; NaN3 .02%
sample_conditions: pH: 5.0; temperature: 293 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
not available | not available | not available | sample_conditions |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 600 MHz
Related Database Links:
BMRB | 11147 11532 15148 15149 15502 18709 |
PDB | |
DBJ | BAA09924 BAC29902 BAC34367 BAC34773 BAC38678 |
EMBL | CAA31110 CAA31284 CAA56631 CAA68441 CAA68526 |
GB | AAA20508 AAA31050 AAA40729 AAA57042 AAA64970 |
PIR | S29857 |
REF | NP_001002937 NP_001004034 NP_001075304 NP_001102843 NP_001162380 |
SP | A9RA84 B0CM99 B1MTB0 P07156 P09429 |
TPG | DAA23902 |
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