BMRB Entry 4256
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4256
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Title: 1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from S. cerevisiae in Both Its Free and DNA Bound Forms, and 1H Assignments of the Free DNA
Deposition date: 1998-10-28 Original release date: 1999-11-23
Authors: McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew
Citation: McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew. "1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from S. cerevisiae in Both Its Free and DNA Bound Forms, and 1H Assignments of the Free DNA" J. Biomol. NMR 13, 397-398 (1999).
Assembly members:
MLU1-BOX binding protein, polymer, 135 residues, Formula weight is not available
DNA binding domain of Mbp1, polymer, 13 residues, Formula weight is not available
Natural source: Common Name: Baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota fungi
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
MLU1-BOX binding protein: SNQIYSARYSGVDVYEFIHS
TGSIMKRKKDDWVNATHILK
AANFAKAKRTRILEKEVLKE
THEKVQGGFGKYQGTWVPLN
IAKQLAEKFSVYDQLKPLFD
FTQTDGSASPPPAPKHHHAS
KVDKLAAALEHHHHH
DNA binding domain of Mbp1: TTTGACGCGTCAA
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 156 |
15N chemical shifts | 115 |
1H chemical shifts | 419 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Mbp1(2-124) | 1 |
2 | DNA strand 1 | 2 |
3 | DNA strand 2 | 2 |
Entities:
Entity 1, Mbp1(2-124) 135 residues - Formula weight is not available
1 | SER | ASN | GLN | ILE | TYR | SER | ALA | ARG | TYR | SER | ||||
2 | GLY | VAL | ASP | VAL | TYR | GLU | PHE | ILE | HIS | SER | ||||
3 | THR | GLY | SER | ILE | MET | LYS | ARG | LYS | LYS | ASP | ||||
4 | ASP | TRP | VAL | ASN | ALA | THR | HIS | ILE | LEU | LYS | ||||
5 | ALA | ALA | ASN | PHE | ALA | LYS | ALA | LYS | ARG | THR | ||||
6 | ARG | ILE | LEU | GLU | LYS | GLU | VAL | LEU | LYS | GLU | ||||
7 | THR | HIS | GLU | LYS | VAL | GLN | GLY | GLY | PHE | GLY | ||||
8 | LYS | TYR | GLN | GLY | THR | TRP | VAL | PRO | LEU | ASN | ||||
9 | ILE | ALA | LYS | GLN | LEU | ALA | GLU | LYS | PHE | SER | ||||
10 | VAL | TYR | ASP | GLN | LEU | LYS | PRO | LEU | PHE | ASP | ||||
11 | PHE | THR | GLN | THR | ASP | GLY | SER | ALA | SER | PRO | ||||
12 | PRO | PRO | ALA | PRO | LYS | HIS | HIS | HIS | ALA | SER | ||||
13 | LYS | VAL | ASP | LYS | LEU | ALA | ALA | ALA | LEU | GLU | ||||
14 | HIS | HIS | HIS | HIS | HIS |
Entity 2, DNA strand 1 13 residues - Formula weight is not available
1 | DT | DT | DT | DG | DA | DC | DG | DC | DG | DT | ||||
2 | DC | DA | DA |
Samples:
sample_one: MLU1-BOX binding protein, [U-15N; U-13C], 0.8 mM; DNA binding domain of Mbp11.0 1.68 mM; Na phosphate 40 mM; NaCl 110 mM; D2O 10%; H2O 90%
sample_two: MLU1-BOX binding protein 0.5 mM; DNA binding domain of Mbp11.0 1.68 mM; Na phosphate 40 mM; NaCl 110 mM; D2O 10%; H2O 90%
sample_conditions_one: pH: 7.6; temperature: 288 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
not available | not available | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
Related Database Links:
BMRB | 4254 |
PDB | |
DBJ | GAA22190 |
EMBL | CAA52271 CAA98618 CAY78452 |
GB | AAS64332 AAS64333 AAS64335 AAS64336 AAS64338 |
REF | NP_010227 |
SP | P39678 |
TPG | DAA11800 |
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