BMRB Entry 10008
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10008
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Title: Solution structure of C-terminal fibronectin type III domain of mouse 1700129L13Rik protein
Deposition date: 2005-10-25 Original release date: 2006-11-06
Authors: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of C-terminal fibronectin type III domain of mouse 1700129L13Rik protein" not known ., .-..
Assembly members:
FN3 domain, polymer, 123 residues, 13038.744 Da.
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
FN3 domain: GSSGSSGPGAPSTVRISKNV
DGIHLSWEPPTSPSGNILEY
SAYLAIRTAQMQDNPSQLVF
MRIYCGLKTSCTVTAGQLAN
AHIDYTSRPAIVFRISAKNE
KGYGPATQIRWLQGNSKSGP
SSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 459 |
| 15N chemical shifts | 106 |
| 1H chemical shifts | 737 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FN3 domain | 1 |
Entities:
Entity 1, FN3 domain 123 residues - 13038.744 Da.
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | GLY | ALA | ||||
| 2 | PRO | SER | THR | VAL | ARG | ILE | SER | LYS | ASN | VAL | ||||
| 3 | ASP | GLY | ILE | HIS | LEU | SER | TRP | GLU | PRO | PRO | ||||
| 4 | THR | SER | PRO | SER | GLY | ASN | ILE | LEU | GLU | TYR | ||||
| 5 | SER | ALA | TYR | LEU | ALA | ILE | ARG | THR | ALA | GLN | ||||
| 6 | MET | GLN | ASP | ASN | PRO | SER | GLN | LEU | VAL | PHE | ||||
| 7 | MET | ARG | ILE | TYR | CYS | GLY | LEU | LYS | THR | SER | ||||
| 8 | CYS | THR | VAL | THR | ALA | GLY | GLN | LEU | ALA | ASN | ||||
| 9 | ALA | HIS | ILE | ASP | TYR | THR | SER | ARG | PRO | ALA | ||||
| 10 | ILE | VAL | PHE | ARG | ILE | SER | ALA | LYS | ASN | GLU | ||||
| 11 | LYS | GLY | TYR | GLY | PRO | ALA | THR | GLN | ILE | ARG | ||||
| 12 | TRP | LEU | GLN | GLY | ASN | SER | LYS | SER | GLY | PRO | ||||
| 13 | SER | SER | GLY |
Samples:
sample_1: FN3 domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 200 mM; d-DTT 2 mM; NaN3 0.02%; D2O 10%; H2O 90%
condition_1: ionic strength: 0.22 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121 - processing
NMRView v5.0.4 - data analysis
Kujira v0.8994 - data analysis
CYANA v2.0.17 - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB24953 |
| REF | XP_006514073 XP_011241845 XP_011241846 XP_011241847 XP_011241848 |
| SP | Q9D968 |
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