BMRB Entry 10127
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10127
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Title: Solution structure of the alpha-helical domain from mouse hypothetical PNPase
Deposition date: 2007-04-02 Original release date: 2008-09-02
Authors: Nagata, T.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.
Citation: Nagata, T.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of the alpha-helical domain from mouse hypothetical PNPase" Not known ., .-..
Assembly members:
alpha-helical domain, polymer, 104 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
alpha-helical domain: GSSGSSGPQKIFTPSAEIVK
YTKIIAMEKLYAVFTDYEHD
KVSRDEAVNKIRLDTEEHLK
EKFPEVDQFEIIESFNIVAK
EVFRSIILNEYKRCDGRDSG
PSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 427 |
| 15N chemical shifts | 88 |
| 1H chemical shifts | 676 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hypothetical PNPase | 1 |
Entities:
Entity 1, hypothetical PNPase 104 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | GLN | LYS | ||||
| 2 | ILE | PHE | THR | PRO | SER | ALA | GLU | ILE | VAL | LYS | ||||
| 3 | TYR | THR | LYS | ILE | ILE | ALA | MET | GLU | LYS | LEU | ||||
| 4 | TYR | ALA | VAL | PHE | THR | ASP | TYR | GLU | HIS | ASP | ||||
| 5 | LYS | VAL | SER | ARG | ASP | GLU | ALA | VAL | ASN | LYS | ||||
| 6 | ILE | ARG | LEU | ASP | THR | GLU | GLU | HIS | LEU | LYS | ||||
| 7 | GLU | LYS | PHE | PRO | GLU | VAL | ASP | GLN | PHE | GLU | ||||
| 8 | ILE | ILE | GLU | SER | PHE | ASN | ILE | VAL | ALA | LYS | ||||
| 9 | GLU | VAL | PHE | ARG | SER | ILE | ILE | LEU | ASN | GLU | ||||
| 10 | TYR | LYS | ARG | CYS | ASP | GLY | ARG | ASP | SER | GLY | ||||
| 11 | PRO | SER | SER | GLY |
Samples:
sample_1: alpha-helical domain, [U-13C; U-15N], 1.12 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
NMRView v5.0.4, Johnson - data analysis
CYANA v2.0, Guntert, P. - refinement, structure solution
NMRPipe v2.3, Delaglio, F. - processing
Kujira v0.901, Kobayashi, N. - data analysis
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB23374 BAE25732 BAE28862 BAE36595 BAE38260 |
| EMBL | CAD45436 |
| GB | AAH27228 AAH55826 EDL23813 EDL23814 |
| REF | NP_082145 XP_006514876 XP_011242053 |
| SP | Q8K1R3 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts