BMRB Entry 10247
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10247
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Title: Solution structures of the LIM domain of human NEDD9 interacting protein with calponin homology and LIM domains
Deposition date: 2008-10-24 Original release date: 2009-11-03
Authors: Sato, M.; Tomizawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Sato, M.; Tomizawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the LIM domain of human NEDD9 interacting protein with calponin homology and LIM domains" . ., .-..
Assembly members:
LIM domain, polymer, 82 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
LIM domain: GSSGSSGQHQEAGAGDLCAL
CGEHLYVLERLCVNGHFFHR
SCFRCHTCEATLWPGGYEQH
PGDGHFYCLQHLPQTDSGPS
SG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 312 |
| 15N chemical shifts | 73 |
| 1H chemical shifts | 459 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LIM domain | 1 |
| 2 | ZINC ION No.1 | 2 |
| 3 | ZINC ION No.2 | 2 |
Entities:
Entity 1, LIM domain 82 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLN | HIS | GLN | ||||
| 2 | GLU | ALA | GLY | ALA | GLY | ASP | LEU | CYS | ALA | LEU | ||||
| 3 | CYS | GLY | GLU | HIS | LEU | TYR | VAL | LEU | GLU | ARG | ||||
| 4 | LEU | CYS | VAL | ASN | GLY | HIS | PHE | PHE | HIS | ARG | ||||
| 5 | SER | CYS | PHE | ARG | CYS | HIS | THR | CYS | GLU | ALA | ||||
| 6 | THR | LEU | TRP | PRO | GLY | GLY | TYR | GLU | GLN | HIS | ||||
| 7 | PRO | GLY | ASP | GLY | HIS | PHE | TYR | CYS | LEU | GLN | ||||
| 8 | HIS | LEU | PRO | GLN | THR | ASP | SER | GLY | PRO | SER | ||||
| 9 | SER | GLY |
Entity 2, ZINC ION No.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: LIM domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.01 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio, F - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9295, Kobayashi, N. - data analysis
CYANA v2.0.17, Guentert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB13949 BAB15124 BAB86289 BAD18727 BAH12301 |
| EMBL | CAB59266 |
| GB | AAH09972 AAH42144 AAH52983 AIC63549 EAW48340 |
| REF | NP_001152763 NP_001273542 NP_073602 XP_001090339 XP_001153132 |
| SP | Q8TDZ2 |
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