BMRB Entry 11004
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11004
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Title: NMR Structure of Ebola fusion peptide in SDS micelles at pH 7 PubMed: 17545161
Deposition date: 2007-07-05 Original release date: 2008-06-25
Authors: Freitas, Monica; Gaspar, Luciane; Lorenzoni, Marcos; Almeida, Fabio; Tinoco, Luzineide; Almeida, Marcius; Maia, Lenize; Degreve, Leo; Valente, Ana Paula; Silva, Jerson
Citation: Freitas, Monica; Gaspar, Luciane; Lorenzoni, Marcos; Almeida, Fabio; Tinoco, Luzineide; Almeida, Marcius; Maia, Lenize; Degreve, Leo; Valente, Ana Paula; Silva, Jerson. "Structure of the Ebola fusion peptide in a membrane-mimetic environment and the interaction with lipid rafts" J. Biol. Chem. 282, 27306-27314 (2007).
Assembly members:
fusion peptide, polymer, 16 residues, 1576.854 Da.
Natural source: Common Name: Ebola virus Taxonomy ID: 205488 Superkingdom: Viruses Kingdom: not available Genus/species: Ebola-like viruses Ebola virus sp.
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
fusion peptide: GAAIGLAWIPYFGPAA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 95 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | fusion peptide | 1 |
Entities:
Entity 1, fusion peptide 16 residues - 1576.854 Da.
| 1 | GLY | ALA | ALA | ILE | GLY | LEU | ALA | TRP | ILE | PRO | ||||
| 2 | TYR | PHE | GLY | PRO | ALA | ALA |
Samples:
sample_1: Ebola fusion peptide 2 mM; SDS 400 mM; potassium phosphate 15 mM; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 0.015 M; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking
CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
ProcheckNMR, Laskowski and MacArthur - data analysis
NMR spectrometers:
- Bruker AMX 600 MHz