BMRB Entry 11058
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR11058
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Chemical shift assignment of PACAP21 bound to phospholipid membranes by magic angle spinning solid-state NMR PubMed: 17996724
Deposition date: 2008-11-25 Original release date: 2009-03-18
Authors: Komi, Nobuyasu; Okawa, Kayo; Tateishi, Yukihiro; Shirakawa, Masahiro; Fujiwara, Toshimichi; Akutsu, Hideo
Citation: Komi, Nobuyasu; Okawa, Kayo; Tateishi, Yukihiro; Shirakawa, Masahiro; Fujiwara, Toshimichi; Akutsu, Hideo. "Structural analysis of pituitary adenylate cyclase-activating polypeptides bound to phospholipid membranes by magic angle spinning solid-state NMR." Biochim. Biophys. Acta 1768, 3001-3011 (2007).
Assembly members:
PACAP21, polymer, 21 residues, 2522.9 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PACAP21: HSDGIFTDSYSRYRKQMAVK
X
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 104 |
| 15N chemical shifts | 15 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PACAP21 | 1 |
Entities:
Entity 1, PACAP21 21 residues - 2522.9 Da.
| 1 | HIS | SER | ASP | GLY | ILE | PHE | THR | ASP | SER | TYR | ||||
| 2 | SER | ARG | TYR | ARG | LYS | GLN | MET | ALA | VAL | LYS | ||||
| 3 | LYS_NH2 |
Samples:
sample_1: PACAP21, [U-13C; U-15N], 3.8 mg; DPPC, [U-98% 2H], 43.2 uM; DPPG, [U-98% 2H], 10.8 uM
sample_conditions_213K: pressure: 1 atm; temperature: 213 K
sample_conditions_223K: pressure: 1 atm; temperature: 223 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 13C-13C DARR | sample_1 | isotropic | sample_conditions_223K |
| 2D 15N-13C | sample_1 | isotropic | sample_conditions_213K |
| 2D 15N-13C DARR | sample_1 | isotropic | sample_conditions_213K |
| 2D (CACB)-CA DARR | sample_1 | isotropic | sample_conditions_213K |
Software:
FELIX v2002, Accelrys Software Inc. - processing
NMR spectrometers:
- Varian Infinity-plus 500 MHz
- Varian Infinity-plus 600 MHz
- Varian Infinity-plus 700 MHz
Related Database Links:
| BMRB | 11059 |
| PDB | |
| DBJ | BAA28355 BAC21153 BAC21154 BAC21155 BAC21156 |
| EMBL | CAA42962 CAA51705 CAA55684 CAA56564 CAG10213 |
| GB | AAA31575 AAA41791 AAB20402 AAB21469 AAB21470 |
| PRF | 2107317A |
| REF | NP_001001544 NP_001009776 NP_001040020 NP_001081947 NP_001093203 |
| SP | O70176 P0DJ95 P13589 P16613 P18509 |
| TPG | DAA15829 |