BMRB Entry 11085
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11085
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Title: Solution structure of the CH domain of human NEDD9 interacting protein with calponin homology and LIM domains
Deposition date: 2009-12-28 Original release date: 2011-01-04
Authors: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the CH domain of human NEDD9 interacting protein with calponin homology and LIM domains" . ., .-..
Assembly members:
CH domain, polymer, 116 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
CH domain: GSSGSSGTQEELLRWCQEQT
AGYPGVHVSDLSSSWADGLA
LCALVYRLQPGLLEPSELQG
LGALEATAWALKVAENELGI
TPVVSAQAVVAGSDPLGLIA
YLSHFHSAFKSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 472 |
| 15N chemical shifts | 112 |
| 1H chemical shifts | 740 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CH domain | 1 |
Entities:
Entity 1, CH domain 116 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | THR | GLN | GLU | ||||
| 2 | GLU | LEU | LEU | ARG | TRP | CYS | GLN | GLU | GLN | THR | ||||
| 3 | ALA | GLY | TYR | PRO | GLY | VAL | HIS | VAL | SER | ASP | ||||
| 4 | LEU | SER | SER | SER | TRP | ALA | ASP | GLY | LEU | ALA | ||||
| 5 | LEU | CYS | ALA | LEU | VAL | TYR | ARG | LEU | GLN | PRO | ||||
| 6 | GLY | LEU | LEU | GLU | PRO | SER | GLU | LEU | GLN | GLY | ||||
| 7 | LEU | GLY | ALA | LEU | GLU | ALA | THR | ALA | TRP | ALA | ||||
| 8 | LEU | LYS | VAL | ALA | GLU | ASN | GLU | LEU | GLY | ILE | ||||
| 9 | THR | PRO | VAL | VAL | SER | ALA | GLN | ALA | VAL | VAL | ||||
| 10 | ALA | GLY | SER | ASP | PRO | LEU | GLY | LEU | ILE | ALA | ||||
| 11 | TYR | LEU | SER | HIS | PHE | HIS | SER | ALA | PHE | LYS | ||||
| 12 | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: CH domain, [U-100% 13C; U-100% 15N], 1.22 mM; TRIS, [U-100% 2H], 20 mM; sodium chloride 100 mM; DTT, [U-100% 2H], 1 mM; sodium azide 0.02%; D2O 10%; H2O 90%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 1H-13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9295, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB13949 BAB15124 BAB86289 BAD18727 BAH12301 |
| EMBL | CAB59266 |
| GB | AAH09972 AAH42144 AAH52983 AIC63549 EAW48341 |
| REF | NP_001152763 NP_001273542 NP_073602 XP_011905848 XP_011905849 |
| SP | Q8TDZ2 |
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