BMRB Entry 11106
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11106
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Title: The solution structure of the FN3 domain of human Midline 2 protein
Deposition date: 2010-02-18 Original release date: 2011-02-18
Authors: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "The solution structure of the FN3 domain of human Midline 2 protein" . ., .-..
Assembly members:
fn3 domain, polymer, 127 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
fn3 domain: GSSGSSGEGLDYLTAPNPPS
IREELCTASHDTITVHWISD
DEFSISSYELQYTIFTGQAN
FISLYNSVDSWMIVPNIKQN
HYTVHGLQSGTRYIFIVKAI
NQAGSRNSEPTRLKTNSQPF
KSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 539 |
| 15N chemical shifts | 127 |
| 1H chemical shifts | 822 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | fn3 domain | 1 |
Entities:
Entity 1, fn3 domain 127 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLU | GLY | LEU | ||||
| 2 | ASP | TYR | LEU | THR | ALA | PRO | ASN | PRO | PRO | SER | ||||
| 3 | ILE | ARG | GLU | GLU | LEU | CYS | THR | ALA | SER | HIS | ||||
| 4 | ASP | THR | ILE | THR | VAL | HIS | TRP | ILE | SER | ASP | ||||
| 5 | ASP | GLU | PHE | SER | ILE | SER | SER | TYR | GLU | LEU | ||||
| 6 | GLN | TYR | THR | ILE | PHE | THR | GLY | GLN | ALA | ASN | ||||
| 7 | PHE | ILE | SER | LEU | TYR | ASN | SER | VAL | ASP | SER | ||||
| 8 | TRP | MET | ILE | VAL | PRO | ASN | ILE | LYS | GLN | ASN | ||||
| 9 | HIS | TYR | THR | VAL | HIS | GLY | LEU | GLN | SER | GLY | ||||
| 10 | THR | ARG | TYR | ILE | PHE | ILE | VAL | LYS | ALA | ILE | ||||
| 11 | ASN | GLN | ALA | GLY | SER | ARG | ASN | SER | GLU | PRO | ||||
| 12 | THR | ARG | LEU | LYS | THR | ASN | SER | GLN | PRO | PHE | ||||
| 13 | LYS | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: fn3 domain, [U-13C; U-15N], 1.2 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-SEPARATED NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-SEPARATED NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMNMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.955, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 700 MHz
Related Database Links:
| PDB | |
| EMBL | CAB56170 |
| GB | AAF07340 AAF07341 AAH17707 AAI40362 AAI46505 |
| REF | NP_035975 NP_438112 XP_001091577 XP_001144794 XP_001491041 |
| SP | Q9QUS6 |
| TPG | DAA13074 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts