BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15267

Title: NMR structure of the anticoccidial peptide PW2 in DPC micelles   PubMed: 17927950

Deposition date: 2007-05-28 Original release date: 2007-10-29

Authors: Almeida, Fabio; Tinoco, Luzineide; Gomes-Neto, Francisco; Valente, Ana Paula

Citation: Cruzeiro-Silva, C.; Gomes-Neto, Francisco; Tinoco, Luzineide; Cilli, E.; Barros, P.; Lapido-Loureiro, P.; Bisch, P.; Almeida, Fabio; Valente, Ana Paula. "Structural biology of membrane-acting peptides: Conformational plasticity of anticoccidial peptide PW2 probed by solution NMR"  Biochim. Biophys. Acta. 1768, 3182-3192 (2007).

Assembly members:
pw2, polymer, 12 residues, 1601 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
pw2: HPLKQYWWRPSI

Data sets:
Data typeCount
1H chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PW21

Entities:

Entity 1, PW2 12 residues - 1601 Da.

1   HISPROLEULYSGLNTYRTRPTRPARGPRO
2   SERILE

Samples:

sample_1: pw2 2 mM; phosphate buffer 20 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CNSSOLVE v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 400 MHz

Related Database Links:

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