BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15500

Title: Solution Structure of the EDA-ID-related C417F mutant of human NEMO zinc finger   PubMed: 18313693

Deposition date: 2007-09-28 Original release date: 2008-03-13

Authors: Cordier, Florence; Vinolo, Emilie; Veron, Michel; Delepierre, Muriel; Agou, Fabrice

Citation: Cordier, Florence; Vinolo, Emilie; Veron, Michel; Delepierre, Muriel; Agou, Fabrice. "Solution Structure of NEMO Zinc Finger and Impact of an Anhidrotic Ectodermal Dysplasia with Immunodeficiency-related Point Mutation"  J. Mol. Biol. 377, 1419-1432 (2008).

Assembly members:
ZN, non-polymer, 65.409 Da.
NEMO_ZF-C417F, polymer, 28 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
NEMO_ZF-C417F: SSDFCCPKCQYQAPDMDTLQ IHVMEFIE

Data sets:
Data typeCount
1H chemical shifts179

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ZINC ION1
2NEMO ZF-C417F2

Entities:

Entity 1, ZINC ION - Zn - 65.409 Da.

1   ZN

Entity 2, NEMO ZF-C417F 28 residues - Formula weight is not available

Residues 3-28 corresponds to residues 394-419 of human NEMO zinc finger. Two Pro in the original sequence are replaced by two Ser (residues 1-2). Cys26 (i.e. C417 in human NEMO numbering) is mutated to Phe.

1   SERSERASPPHECYSCYSPROLYSCYSGLN
2   TYRGLNALAPROASPMETASPTHRLEUGLN
3   ILEHISVALMETGLUPHEILEGLU

Samples:

sample_1: NEMO ZF-C417F 1 mM; ZnS04 2 mM; TCEP 2 mM

sample_conditions_1: ionic strength: 0 M; pH: 6.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D purged-COSYsample_1isotropicsample_conditions_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v2.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v5.03, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

ARIA v1.2, Linge, O, . - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

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