BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15649

Title: 1H chemical shift and NMR structure of Herpes simplex virus type 1 (HSV-1)fusion peptide L627S gH626-644 mutation L(627)S   PubMed: 18678872

Deposition date: 2008-02-03 Original release date: 2008-08-22

Authors: Raiola, Luca; Falanga, Annarita; Isernia, Carla; Galdiero, Stefania; Fattorusso, Roberto; Pedone, Carlo

Citation: Galdiero, Stefania; Falanga, Annarita; Vitiello, Mariateresa; Raiola, Luca; Fattorusso, Roberto; Browne, Helena; Pedone, Carlo; Isernia, Carla; Galdiero, Massimiliano. "Analysis of a membrane interacting region of herpes simplex virus type 1 glycoprotein H"  J. Biol. Chem. 283, 29993-30009 (2008).

Assembly members:
HSV-1_gH_protein,_L627S_gH626-644_fusion_peptide, polymer, 19 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 10298   Superkingdom: Viruses   Kingdom: not available   Genus/species: Simplexvirus Human herpesvirus 1

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
HSV-1_gH_protein,_L627S_gH626-644_fusion_peptide: GSASTLTRWAHYNALIRAF

Data sets:
Data typeCount
1H chemical shifts130

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HSV-1_gH_protein,_L627S_gH626-644_fusion_peptide1

Entities:

Entity 1, HSV-1_gH_protein,_L627S_gH626-644_fusion_peptide 19 residues - Formula weight is not available

1   GLYSERALASERTHRLEUTHRARGTRPALA
2   HISTYRASNALALEUILEARGALAPHE

Samples:

L627S_gH626-644: TFE, [U-99% 2H], 80 v/v; H2O 20 v/v; L627S gH626-644 2 mM

L627S_gH626-644_conditions: pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYL627S_gH626-644isotropicL627S_gH626-644_conditions
2D DQF-COSYL627S_gH626-644isotropicL627S_gH626-644_conditions
2D 1H-1H NOESYL627S_gH626-644isotropicL627S_gH626-644_conditions

Software:

XEASY, Bartels et al. - chemical shift assignment, structure solution

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz