BMRB Entry 15666
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15666
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Title: Mutant Collision E7 protein IM7 with an extended helix III in M Urea PubMed: 19651139
Deposition date: 2008-02-20 Original release date: 2009-11-10
Authors: Figueiredo, Angelo; Whittaker, Sara; Knowling, Stuart; Radford, Sheena; Moore, Geoffrey
Citation: Knowling, Stuart; Figueiredo, Angelo Miguel; Whittaker, Sara B-M; Moore, Geoffrey; Radford, Sheena. "Amino acid insertion reveals a necessary three-helical intermediate in the folding pathway of the colicin E7 immunity protein Im7." J. Mol. Biol. 392, 1074-1086 (2009).
Assembly members:
Im7_mutant, polymer, 101 residues, Formula weight is not available
Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Im7_mutant: MEHHHHHHELKNSISDYTEA
EFVQLLKEIEKENVAATDDV
LDVLLEHFVKITEHPDGTAL
IYEAAARAAANPGGDGGGPE
GIVKEIKEWRAANGKPGFKQ
G
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 175 |
15N chemical shifts | 88 |
1H chemical shifts | 88 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Im7 mutant | 1 |
Entities:
Entity 1, Im7 mutant 101 residues - Formula weight is not available
1 | MET | GLU | HIS | HIS | HIS | HIS | HIS | HIS | GLU | LEU | ||||
2 | LYS | ASN | SER | ILE | SER | ASP | TYR | THR | GLU | ALA | ||||
3 | GLU | PHE | VAL | GLN | LEU | LEU | LYS | GLU | ILE | GLU | ||||
4 | LYS | GLU | ASN | VAL | ALA | ALA | THR | ASP | ASP | VAL | ||||
5 | LEU | ASP | VAL | LEU | LEU | GLU | HIS | PHE | VAL | LYS | ||||
6 | ILE | THR | GLU | HIS | PRO | ASP | GLY | THR | ALA | LEU | ||||
7 | ILE | TYR | GLU | ALA | ALA | ALA | ARG | ALA | ALA | ALA | ||||
8 | ASN | PRO | GLY | GLY | ASP | GLY | GLY | GLY | PRO | GLU | ||||
9 | GLY | ILE | VAL | LYS | GLU | ILE | LYS | GLU | TRP | ARG | ||||
10 | ALA | ALA | ASN | GLY | LYS | PRO | GLY | PHE | LYS | GLN | ||||
11 | GLY |
Samples:
sample_1: Im7 mutant, [U-13C; U-15N], 1 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 50 mM; pH*: 7; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D CBCANH | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNN | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView v5.1.1, Johnson, One Moon Scientific - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance 800 MHz
- Varian INOVA 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts