BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16122

Title: HCV NS4B(227-254)   PubMed: 19357161

Deposition date: 2009-01-15 Original release date: 2009-04-17

Authors: Montserret, Roland; Penin, Francois

Citation: Gouttenoire, Jerome; Castet, Valerie; Montserret, Roland; Arora, Naveen; Raussens, Vincent; Ruysschaert, Jean-Marie; Diesis, Eric; Blum, Hubert; Penin, Francois; Moradpour, Darius. "Identification of a Novel Determinant for Membrane Association in Hepatitis C Virus Nonstructural Protein 4B"  J. Virol 83, 6257-6268 (2009).

Assembly members:
HCV NS4B(227-254), polymer, 28 residues, 3111.629 Da.

Natural source:   Common Name: Hepatitis C   Taxonomy ID: 11103   Superkingdom: Viruses   Kingdom: not available   Genus/species: Hepacivirus Hepatitis C Virus

Experimental source:   Production method: chemical synthesis   Host organism: none

Entity Sequences (FASTA):
HCV NS4B(227-254): SDAAARVTAILSSLTVTQLL RRLHQWIS

Data sets:
Data typeCount
13C chemical shifts82
1H chemical shifts177

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HCV NS4B(227-254)1

Entities:

Entity 1, HCV NS4B(227-254) 28 residues - 3111.629 Da.

1   SERASPALAALAALAARGVALTHRALAILE
2   LEUSERSERLEUTHRVALTHRGLNLEULEU
3   ARGARGLEUHISGLNTRPILESER

Samples:

sample_1: entity 2 mM; H2O 50%; trifluoroethanol 50%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
GB AAA45534 AAA45676 AAA45677 AAB66324 AAB67036
REF NP_671491 NP_751926
SP P26664 P27958