BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16550

Title: Specific motifs of the V-ATPase a2-subunit isoform interact with catalytic and regulatory domains of ARNO   PubMed: 20153292

Deposition date: 2009-10-11 Original release date: 2010-02-18

Authors: Merkulova, Maria; Bakulina, Anastasia; Thaker, Youg; Gruber, Gerhard; Marshansky, Vladimir

Citation: Merkulova, Maria; Bakulina, Anastasia; Thaker, Youg Raj; Gruber, Gerhard; Marshansky, Vladimir. "Specific motifs of the V-ATPase a2-subunit isoform interact with catalytic and regulatory domains of ARNO."  Biochim. Biophys. Acta 1797, 1398-1409 (2010).

Assembly members:
ARNO(375-400), polymer, 26 residues, 3076 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
ARNO(375-400): VSVDPFYEMLAARKKRISVK KKQEQP

Data sets:
Data typeCount
1H chemical shifts156

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PB domain1

Entities:

Entity 1, PB domain 26 residues - 3076 Da.

Residues from 375-400 represents PB (Polybasic)of ARNO protein.

1   VALSERVALASPPROPHETYRGLUMETLEU
2   ALAALAARGLYSLYSARGILESERVALLYS
3   LYSLYSGLNGLUGLNPRO

Samples:

sample_1: ARNO(375-400), [U-100% 15N], 1-2 mM

sample_conditions_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY v2.6.9, Goddard - chemical shift assignment, chemical shift calculation, data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAA33429 BAA33430 BAA33431 BAA33432 BAA87927
EMBL CAA68084
GB AAB09591 AAB41444 AAC77924 AAH04361 AAH04662
REF NP_001070562 NP_001106171 NP_001135984 NP_001137592 NP_001247820
SP P63034 P63035 Q2KI41 Q76MY7 Q99418
TPG DAA19658