BMRB Entry 16770
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PDB ID: 2kv7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16770
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Title: NMR solution structure of a soluble PrgI mutant from Salmonella Typhimurium PubMed: 20543831
Deposition date: 2010-03-09 Original release date: 2010-07-27
Authors: Schmidt, Holger; Poyraz, Oemer; Seidel, Karsten; Delissen, Friedmar; Ader, Christian; Tenenboim, Hezi; Goosmann, Christian; Laube, Britta; Thuenemann, Andreas; Zychlinski, Arturo; Baldus, Marc; Lange, Adam; Griesinger, Christian; Kolbe, Michael
Citation: Poyraz, Omer; Schmidt, Holger; Seidel, Karsten; Delissen, Friedmar; Ader, Christian; Tenenboim, Hezi; Goosmann, Christian; Laube, Britta; Thunemann, Andreas; Zychlinsky, Arturo; Baldus, Marc; Lange, Adam; Griesinger, Christian; Kolbe, Michael. "Protein refolding is required for assembly of the type three secretion needle." Nat. Struct. Mol. Biol. 17, 788-792 (2010).
Assembly members:
PrgI, polymer, 83 residues, 9091.127 Da.
Natural source: Common Name: Salmonella enterica Taxonomy ID: 28901 Superkingdom: Bacteria Kingdom: not available Genus/species: Salmonella typhimurium
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PrgI: GSHMATPWSGYLDDVSAKFD
TGVDNLQTQVTEALDKLAAK
PSDPALLAAYQSKLSEYNLY
RNAQSNTAKAFKDIDAAIIQ
NFR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 335 |
| 15N chemical shifts | 89 |
| 1H chemical shifts | 568 |
Additional metadata:
Related Database Links:
| BMRB | 18276 |
| PDB | 2KV7 2LPZ 2MEX 2X9C 3ZQB 3ZQE |
| DBJ | BAJ37865 BAP08778 |
| EMBL | CAR34293 CAR38585 CBG25842 CBW18951 CCF89508 |
| GB | AAB60189 AAL21753 AAX49613 AAX66711 ACF64232 |
| REF | NP_461794 WP_000235228 YP_009073587 |
| SP | P41784 |
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