BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16856

Title: Solution Structure of acyl carrier protein from Borrelia burgdorferi, Seattle Structural Genomics Center for Infectious Disease (SSGCID) consortium

Deposition date: 2010-04-12 Original release date: 2010-04-20

Authors: Barnwal, Ravi Pratap; Vittal, Vinayak; Moody, James; Varani, Gabriele

Citation: Barnwal, Ravi Pratap; Vittal, Vinayak; Moody, James; Varani, Gabriele. "NMR Structure of acyl carrier protein from Borrelia burgdorferi."  Not known ., .-..

Assembly members:
acyl_carrier_protein, polymer, 84 residues, 9642.646 Da.

Natural source:   Common Name: Lyme disease spirochete   Taxonomy ID: 139   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Borrelia burgdorferi

Experimental source:   Production method: recombinant technology   Host organism: Borrelia burgdorferi

Entity Sequences (FASTA):
acyl_carrier_protein: GPGSMDNDEIFSKVRSIISE QLDKKEDEITTDSRFVEDLN ADSLDIYELLYLLEEAFDDK IPENEANEFETVGDVVNFIK KRKG

Data sets:
Data typeCount
13C chemical shifts335
15N chemical shifts90
1H chemical shifts582

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 84 residues - 9642.646 Da.

1   GLYPROGLYSERMETASPASNASPGLUILE
2   PHESERLYSVALARGSERILEILESERGLU
3   GLNLEUASPLYSLYSGLUASPGLUILETHR
4   THRASPSERARGPHEVALGLUASPLEUASN
5   ALAASPSERLEUASPILETYRGLULEULEU
6   TYRLEULEUGLUGLUALAPHEASPASPLYS
7   ILEPROGLUASNGLUALAASNGLUPHEGLU
8   THRVALGLYASPVALVALASNPHEILELYS
9   LYSARGLYSGLY

Samples:

sample_1: acyl carrier protein (BobuA.00658.a), [U-95% 13C; U-95% 15N], 1.1 mM

sample_2: acyl carrier protein (BobuA.00658.a), [U-95% 15N], 1.1 mM

sample_3: acyl carrier protein (BobuA.00658.a) 1.1 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

PDB
GB AAC67041 AAU07553 ABH01977 ACK74653 ADQ29392
REF NP_212838 WP_002557291 WP_002656801 WP_004789379 WP_006068702
SP B7J0F1 O51647 Q0SMF1 Q660G8

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts