BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17003

Title: Vpr(1-20) Vpr(21-40) Vpr(25-40)   PubMed: 20920334

Deposition date: 2010-06-16 Original release date: 2010-10-14

Authors: Fossen, Torgils; Solbak, Sara

Citation: Solbak, Sara; Reksten, Tove; Wray, Victor; Bruns, Karsten; Horvli, Ole; Raae, Arnt; Henklein, Petra; Henklein, Peter; Roder, Rene; Mitzner, David; Schubert, Ulrich; Fossen, Torgils. "The intriguing Cyclophilin A-HIV-1 Vpr interaction: prolyl cis/trans isomerisation catalysis and specific binding."  BMC Struct. Biol. 10, 31-31 (2010).

Assembly members:
N-terminal_Vpr_peptides, polymer, 40 residues, Formula weight is not available

Natural source:   Common Name: HIV-1   Taxonomy ID: 11676   Superkingdom: virus   Kingdom: not available   Genus/species: Lentivirus HIV

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
N-terminal_Vpr_peptides: MEQAPEDQGPQREPYNEWTL ELLEELKSEAVRHFPRIWLH

Data sets:
Data typeCount
1H chemical shifts316

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Vpr(1-40)1
2Vpr(25-40)1

Entities:

Entity 1, Vpr(1-40) 40 residues - Formula weight is not available

1   METGLUGLNALAPROGLUASPGLNGLYPRO
2   GLNARGGLUPROTYRASNGLUTRPTHRLEU
3   GLULEULEUGLUGLULEULYSSERGLUALA
4   VALARGHISPHEPROARGILETRPLEUHIS

Samples:

sample_1: N-terminal Vpr peptides1 – 2 mM; Aqueous Phosphate Buffer 90%; D2O 10%

sample_conditions_1: ionic strength: 0.050 M; pH: 7; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H ROESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

DBJ BAA93966 BAA93967 BAA93972 BAA93973 BAA93975
EMBL CAA06949 CAA92863 CAA92864 CAA92870 CAA92871
GB AAA21763 AAA44871 AAA44983 AAA44990 AAA45055
SP P05954 P12519 P12520 P19555 P20883