BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17040

Title: STRUCTURAL AND FUNCTIONAL ANALYSIS OF TM VI OF THE NHE1 ISOFORM OF THE Na+/H+ EXCHANGER   PubMed: 20843797

Deposition date: 2010-07-01 Original release date: 2010-09-03

Authors: Tzeng, Jennifer; Lee, Brian; Sykes, Brian; Fliegel, Larry

Citation: Tzeng, Jennifer; Lee, Brian; Sykes, Brian; Fliegel, Larry. "Structural and Functional Analysis of Transmembrane Segment VI of the NHE1 Isoform of the Na+/H+ Exchanger."  J. Biol. Chem. 285, 36656-36665 (2010).

Assembly members:
TM_VI, polymer, 32 residues, 3479.16 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
TM_VI: XKKDNLLFGSIISAVDPVAV LAVFEEIHKKKX

Data sets:
Data typeCount
1H chemical shifts216

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TM VI1

Entities:

Entity 1, TM VI 32 residues - 3479.16 Da.

N-terminal acetyl, C-terminal NH2 capped polypeptide lysine tagged termini

1   ALYLYSLYSASPASNLEULEUPHEGLYSER
2   ILEILESERALAVALASPPROVALALAVAL
3   LEUALAVALPHEGLUGLUILEHISLYSLYS
4   LYSNH2

Samples:

sample_1: TM VI 2 mM; DSS 0.25 mM; DPC, [U-2H], 150 mM

sample_conditions_1: ionic strength: 0 M; pH: 5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak assignment, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 19484
PDB
DBJ BAE02258 BAE75800
EMBL CAA42558 CAA69925 CAF94405 CAG03374 CDQ60725
GB AAA31092 AAB20633 AAB59460 AAC60606 AAF21350
REF NP_001007104 NP_001038108 NP_001081553 NP_001095191 NP_001273957
SP P19634 P23791 P48762