BMRB Entry 17068
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17068
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Title: Assigment of the 1H, 13C, and 15N resonances of the yeast frataxin (Yfh1) under cold denaturation PubMed: 20979399
Deposition date: 2010-07-21 Original release date: 2010-11-09
Authors: Adrover, Miquel; Pastore, Annalisa; Temussi, Piero Andrea
Citation: Adrover, Miquel; Esposito, Veronica; Martorell, Gabriel; Pastore, Annalisa; Temussi, Piero Andrea. "Understanding cold denaturation: the case study of yfh1." J. Am. Chem. Soc. 132, 16240-16246 (2010).
Assembly members:
Yeast_frataxin_(Yfh1), polymer, 123 residues, 13783.4 Da.
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: cell free synthesis Host organism: Escherichia coli
Entity Sequences (FASTA):
Yeast_frataxin_(Yfh1): MESSTDGQVVPQEVLNLPLE
KYHEEADDYLDHLLDSLEEL
SEAHPDCIPDVELSHGVMTL
EIPAFGTYVINKQPPNKQIW
LASPLSGPNRFDLLNGEWVS
LRNGTKLTDILTEEVEKAIS
KSQ
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 210 |
15N chemical shifts | 92 |
1H chemical shifts | 592 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Frataxin | 1 |
Entities:
Entity 1, Frataxin 123 residues - 13783.4 Da.
1 | MET | GLU | SER | SER | THR | ASP | GLY | GLN | VAL | VAL | ||||
2 | PRO | GLN | GLU | VAL | LEU | ASN | LEU | PRO | LEU | GLU | ||||
3 | LYS | TYR | HIS | GLU | GLU | ALA | ASP | ASP | TYR | LEU | ||||
4 | ASP | HIS | LEU | LEU | ASP | SER | LEU | GLU | GLU | LEU | ||||
5 | SER | GLU | ALA | HIS | PRO | ASP | CYS | ILE | PRO | ASP | ||||
6 | VAL | GLU | LEU | SER | HIS | GLY | VAL | MET | THR | LEU | ||||
7 | GLU | ILE | PRO | ALA | PHE | GLY | THR | TYR | VAL | ILE | ||||
8 | ASN | LYS | GLN | PRO | PRO | ASN | LYS | GLN | ILE | TRP | ||||
9 | LEU | ALA | SER | PRO | LEU | SER | GLY | PRO | ASN | ARG | ||||
10 | PHE | ASP | LEU | LEU | ASN | GLY | GLU | TRP | VAL | SER | ||||
11 | LEU | ARG | ASN | GLY | THR | LYS | LEU | THR | ASP | ILE | ||||
12 | LEU | THR | GLU | GLU | VAL | GLU | LYS | ALA | ILE | SER | ||||
13 | LYS | SER | GLN |
Samples:
sample_1: Yeast frataxin (Yfh1), [U-100% 13C; U-100% 15N], 0.4 0.5 mM; D2O 90 ± 2 %; H2O 10 ± 2 %; DTT 1 ± 0.2 mM; HEPES 20 ± 1 mM
sample_conditions_1: ionic strength: 0.004 M; pH: 7.0; pressure: 1 atm; temperature: 272.0 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY v1.3.2, Bartels et al., Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, peak picking, processing
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
BMRB | 6356 17641 19991 |
PDB | |
DBJ | GAA22128 |
EMBL | CAA98688 CAY78388 |
GB | AAS56486 AHY74893 AJP37633 AJU57746 AJU58449 |
REF | NP_010163 |
SP | Q07540 |
TPG | DAA11740 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts