BMRB Entry 17133
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17133
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Title: Chemical shift assignments for the RanBP2-type ZnF from Ewing's Sarcoma (EWS) PubMed: 21256132
Deposition date: 2010-08-18 Original release date: 2011-01-25
Authors: Leung, Wilfred; Mansfield, Robyn
Citation: Nguyen, Cuong; Mansfield, Robyn; Leung, Wilfred; Vaz, Paula; Loughlin, Fionna; Grant, Richard; Mackay, Joel. "Characterization of a Family of RanBP2-Type Zinc Fingers that Can Recognize Single-Stranded RNA." J. Mol. Biol. 407, 273-283 (2011).
Assembly members:
EWS, polymer, 39 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
EWS: GSNVQHRAGDWQCPNPGCGN
QNFAWRTECNQCKAPKPEG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 120 |
| 15N chemical shifts | 38 |
| 1H chemical shifts | 162 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EWS | 1 |
| 2 | ZN | 2 |
Entities:
Entity 1, EWS 39 residues - Formula weight is not available
| 1 | GLY | SER | ASN | VAL | GLN | HIS | ARG | ALA | GLY | ASP | ||||
| 2 | TRP | GLN | CYS | PRO | ASN | PRO | GLY | CYS | GLY | ASN | ||||
| 3 | GLN | ASN | PHE | ALA | TRP | ARG | THR | GLU | CYS | ASN | ||||
| 4 | GLN | CYS | LYS | ALA | PRO | LYS | PRO | GLU | GLY |
Entity 2, ZN - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: EWS, [U-100% 13C; U-100% 15N], 0.5 mM; D2O 5%; DSS 20 uM; sodium chloride 100 mM; sodium phosphate 25 mM; DTT 1 mM; H2O 95%
sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
TOPSPIN, Bruker Biospin - processing
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| DBJ | BAB29301 BAB31732 BAB71145 BAB71252 BAE02261 |
| EMBL | CAA47350 CAA51489 CAA55815 CAA69177 CAG30376 |
| GB | AAH04817 AAH11048 AAH63928 AAH68226 AAH72442 |
| PRF | 1818357A |
| REF | NP_001020803 NP_001089902 NP_001103270 NP_001156757 NP_001156758 |
| SP | Q01844 Q61545 |
| TPG | DAA20531 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts