BMRB Entry 17228
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17228
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Title: C-terminal zinc knuckle of the HIVNCp7 PubMed: 22303928
Deposition date: 2010-10-01 Original release date: 2012-03-09
Authors: Quintal, Susana; Viegas, Aldino; Cabrita, Eurico; Farrell, Nicholas; Erhardt, Stefan
Citation: Quintal, Susana; Viegas, Aldino; Erhardt, Stefan; Cabrita, Eurico; Farrell, Nicholas. "Platinated DNA Affects Zinc Finger Conformation. Interaction of a Platinated Single-Stranded Oligonucleotide and the C-Terminal Zinc Finger of Nucleocapsid Protein HIVNCp7." Biochemistry 51, 1752-1761 (2012).
Assembly members:
C-terminal_zinc_knuckle_of_the_HIVNCp7, polymer, 19 residues, 2230.615 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: HIV Taxonomy ID: 12721 Superkingdom: viruses Kingdom: not available Genus/species: Lentivirus HIV
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
C-terminal_zinc_knuckle_of_the_HIVNCp7: KGCWKCGKEGHQMKDCTER
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 118 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
| 2 | ZINC_ION_1 | 2 |
| 3 | ZINC_ION_2 | 2 |
| 4 | ZINC_ION_3 | 2 |
| 5 | ZINC_ION_4 | 2 |
Entities:
Entity 1, entity 19 residues - 2230.615 Da.
| 1 | LYS | GLY | CYS | TRP | LYS | CYS | GLY | LYS | GLU | GLY | ||||
| 2 | HIS | GLN | MET | LYS | ASP | CYS | THR | GLU | ARG |
Entity 2, ZINC_ION_1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: C-terminal zinc knuckle of the HIVNCp7 1 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 6 mM; pH: 5.7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
SPARKY v3.114, Goddard - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 1656 17216 17229 18980 2217 25532 |
| PDB | |
| DBJ | BAA12988 BAA12996 BAA84661 BAA84662 BAA84669 |
| EMBL | CAA28011 CAA42971 CAA42973 CAA73647 CAA77483 |
| GB | AAA43911 AAA44201 AAA44313 AAA44314 AAA44317 |
| PIR | FOVWLV |
| PRF | 1102247B 1103299C |
| REF | NP_057849 NP_057850 NP_579881 |
| SP | O89939 O89940 P03347 P03348 P03366 |