BMRB Entry 17571
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17571
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Title: (Revised) SOLUTION STRUCTURE OF THE MONOMERIC FORM OF A MUTANT UNLIGANDED BOVINE NEUROPHYSIN, 20 STRUCTURES.
Deposition date: 2011-04-01 Original release date: 2012-04-03
Authors: Lee, Hunjoong; Nguyen, Tam; Bracken, Clay; Breslow, Esther
Citation: Lee, Hunjoong; Naik, Mandar; Bracken, Clay; Breslow, Esther. "Structural Basis of the Dimerization-Induced Increase in Neurophysin-Hormone Affinity: Interplay of Inter-Domain and Inter-Subunit Interactions" Not known ., .-..
Assembly members:
Mutant Unliganded Bovine Neurophysin, polymer, 92 residues, 9286.461 Da.
Natural source: Common Name: cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Mutant Unliganded Bovine Neurophysin: AVLDLDVRTCLPCGPGGKGR
CFGPSICCGDELGCFVGTAE
ALRCQEENYLPSPCQSGQKP
CGSGGRCAAAGICCSPDGCE
EDPACDPEAAFS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 170 |
| 15N chemical shifts | 74 |
| 1H chemical shifts | 367 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Mutant Unliganded Bovine Neurophysin | 1 |
Entities:
Entity 1, Mutant Unliganded Bovine Neurophysin 92 residues - 9286.461 Da.
| 1 | ALA | VAL | LEU | ASP | LEU | ASP | VAL | ARG | THR | CYS | ||||
| 2 | LEU | PRO | CYS | GLY | PRO | GLY | GLY | LYS | GLY | ARG | ||||
| 3 | CYS | PHE | GLY | PRO | SER | ILE | CYS | CYS | GLY | ASP | ||||
| 4 | GLU | LEU | GLY | CYS | PHE | VAL | GLY | THR | ALA | GLU | ||||
| 5 | ALA | LEU | ARG | CYS | GLN | GLU | GLU | ASN | TYR | LEU | ||||
| 6 | PRO | SER | PRO | CYS | GLN | SER | GLY | GLN | LYS | PRO | ||||
| 7 | CYS | GLY | SER | GLY | GLY | ARG | CYS | ALA | ALA | ALA | ||||
| 8 | GLY | ILE | CYS | CYS | SER | PRO | ASP | GLY | CYS | GLU | ||||
| 9 | GLU | ASP | PRO | ALA | CYS | ASP | PRO | GLU | ALA | ALA | ||||
| 10 | PHE | SER |
Samples:
sample_1: entity, [U-100% 15N], 1.4 mM; H2O 90%; D2O 10%
sample_2: entity, [U-100% 13C; U-100% 15N], 1.4 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 283 K
sample_conditions_2: pH: 7.5; pressure: 1 atm; temperature: 298 K
sample_conditions_3: pH: 7.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_2 |
| 3D HNHA | sample_2 | isotropic | sample_conditions_3 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_3 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_3 |
| 2D 1H-15N HSQC | sample_2 | anisotropic | sample_conditions_3 |
Software:
CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMRView, Johnson, One Moon Scientific - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 17561 |
| PDB | |
| DBJ | BAK09299 BAK09301 BAK09303 |
| EMBL | CAA23448 CAA23450 CAA25462 CAJ81049 |
| GB | AAA30680 AAI41998 AFU54450 ELK04017 |
| PRF | 0904308A 1005247A 1307200B |
| REF | NP_789825 XP_006042791 |
| SP | P01175 |
| TPG | DAA23107 |
Download simulated HSQC data in one of the following formats:
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