BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17621

Title: Solution structure of the EF-hand domain of Human Polycystin 2

Deposition date: 2011-05-04 Original release date: 2011-12-20

Authors: Allen, Mark; Qamar, S.; Sandford, R.

Citation: Allen, Mark; Qamar, S.; Sandford, R.. "Solution structure of the EF-hand domain of Human Polycystin 2"  To Be Published ., .-..

Assembly members:
POLYCYSTIN-2, polymer, 79 residues, 9036.6057 Da.
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis   Host organism: unknown

Entity Sequences (FASTA):
POLYCYSTIN-2: GGSLKKNTVDDISESLRQGG GKLNFDELRQDLKGKGHTDA EIEAIFTKYDQDGDQELTEH EHQQMRDDLEKEREDLDLD

Data sets:
Data typeCount
13C chemical shifts152
15N chemical shifts69
1H chemical shifts360

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1POLYCYSTIN-21
2Calcium Ion2

Entities:

Entity 1, POLYCYSTIN-2 79 residues - 9036.6057 Da.

1   GLYGLYSERLEULYSLYSASNTHRVALASP
2   ASPILESERGLUSERLEUARGGLNGLYGLY
3   GLYLYSLEUASNPHEASPGLULEUARGGLN
4   ASPLEULYSGLYLYSGLYHISTHRASPALA
5   GLUILEGLUALAILEPHETHRLYSTYRASP
6   GLNASPGLYASPGLNGLULEUTHRGLUHIS
7   GLUHISGLNGLNMETARGASPASPLEUGLU
8   LYSGLUARGGLUASPLEUASPLEUASP

Entity 2, Calcium Ion - Ca - 40.078 Da.

1   CA

Samples:

sample_1: POLYCYSTIN-2, [U-13C; U-15N], 1.5 mM; H2O 95%; D2O 5%; Potassium phosphate 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 150.000 mM; pH: 6.500; pressure: 1 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1solutionsample_conditions_1
2D TOCSYsample_1solutionsample_conditions_1
2D DQF COSYsample_1solutionsample_conditions_1
2D HSQC 15Nsample_1solutionsample_conditions_1
2D HSQC 13Csample_1solutionsample_conditions_1
3D HNCACBsample_1solutionsample_conditions_1
3D CBCACONHsample_1solutionsample_conditions_1
3D HNCOsample_1solutionsample_conditions_1
3D HNCACOsample_1solutionsample_conditions_1
3D HBHACONHsample_1solutionsample_conditions_1
3D HNHBsample_1solutionsample_conditions_1

Software:

AutoDep v4.3, PDBe - collection

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

CNS v1.2, BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP PKD2_HUMAN
BMRB 16191 16590 18268
PDB
DBJ BAG56956 BAG57494
EMBL CAG31243 CAI38797
GB AAC16004 AAC50520 AAC50933 AAI11455 AAI12262
REF NP_000288 NP_001026311 NP_001039777 NP_001232908 XP_001099242
SP Q13563 Q4GZT3
TPG DAA28806

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts