BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17719

Title: Solution structure of esophageal cancer-related gene 2   PubMed: 22528291

Deposition date: 2011-06-20 Original release date: 2012-05-09

Authors: Feng, Yingang; Geng, Yong; Wang, Jinfeng

Citation: Feng, Yingang; Geng, Yong; Zhou, Tao; Wang, Jinfeng. "NMR structure note: human esophageal cancer-related gene 2."  J. Biomol. NMR 53, 65-70 (2012).

Assembly members:
esophageal cancer-related gene 2, polymer, 66 residues, 7209.305 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
esophageal cancer-related gene 2: SEAASLSPKKVDCSIYKKYP VVAIPCPITYLPVCGSDYIT YGNECHLCTESLKSNGRVQF LHDGSC

Data sets:
Data typeCount
13C chemical shifts289
15N chemical shifts64
1H chemical shifts446

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1esophageal cancer-related gene 21

Entities:

Entity 1, esophageal cancer-related gene 2 66 residues - 7209.305 Da.

1   SERGLUALAALASERLEUSERPROLYSLYS
2   VALASPCYSSERILETYRLYSLYSTYRPRO
3   VALVALALAILEPROCYSPROILETHRTYR
4   LEUPROVALCYSGLYSERASPTYRILETHR
5   TYRGLYASNGLUCYSHISLEUCYSTHRGLU
6   SERLEULYSSERASNGLYARGVALGLNPHE
7   LEUHISASPGLYSERCYS

Samples:

sample_1: sodium acetate, [U-2H], 50 mM; potassium chloride 100 mM; DSS 0.01%; sodium azide 0.01%; EC-RG2, [U-13C; U-15N], 0.5 – 1.0 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 5.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

FELIX, Accelrys Software Inc. - chemical shift assignment, data analysis, refinement

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
GB AAI09386 AAI10068 AAK27795 AAQ89431 AIC52595
REF NP_115955 XP_001103960 XP_001160972 XP_002816126 XP_003266657
SP P58062

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts