BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17745

Title: NMR Assignment of PFV RNase H domain   PubMed: 22325739

Deposition date: 2011-06-29 Original release date: 2012-03-27

Authors: Leo, Berit; Schweimer, Kristian; Woehrl, Birgitta

Citation: Leo, Berit; Hartl, Maximilian; Schweimer, Kristian; Mayr, Florian; Wohrl, Birgitta. "Insights into the structure and activity of prototype foamy virus RNase H."  Retrovirology 9, 14-14 (2012).

Assembly members:
PFV_RNase_H, polymer, 165 residues, Formula weight is not available

Natural source:   Common Name: prototype foamy virus   Taxonomy ID: not available   Superkingdom: Viruses   Kingdom: not available   Genus/species: foamy virus prototype foamy virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PFV_RNase_H: GAMGQYEGVFYTDGSAIKSP DPTKSNNAGMGIVHATYKPE YQVLNQWSIPLGNHTAQMAE IAAVEFACKKALKIPGPVLV ITDSFYVAESANKELPYWKS NGFVNNKKKPLKHISKWKSI AECLSMKPDITIQHEKGHQP TNTSIHTEGNALADKLATQG SYVVN

Data sets:
Data typeCount
13C chemical shifts600
15N chemical shifts142
1H chemical shifts1006

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PFV RNase H1

Entities:

Entity 1, PFV RNase H 165 residues - Formula weight is not available

1   GLYALAMETGLYGLNTYRGLUGLYVALPHE
2   TYRTHRASPGLYSERALAILELYSSERPRO
3   ASPPROTHRLYSSERASNASNALAGLYMET
4   GLYILEVALHISALATHRTYRLYSPROGLU
5   TYRGLNVALLEUASNGLNTRPSERILEPRO
6   LEUGLYASNHISTHRALAGLNMETALAGLU
7   ILEALAALAVALGLUPHEALACYSLYSLYS
8   ALALEULYSILEPROGLYPROVALLEUVAL
9   ILETHRASPSERPHETYRVALALAGLUSER
10   ALAASNLYSGLULEUPROTYRTRPLYSSER
11   ASNGLYPHEVALASNASNLYSLYSLYSPRO
12   LEULYSHISILESERLYSTRPLYSSERILE
13   ALAGLUCYSLEUSERMETLYSPROASPILE
14   THRILEGLNHISGLULYSGLYHISGLNPRO
15   THRASNTHRSERILEHISTHRGLUGLYASN
16   ALALEUALAASPLYSLEUALATHRGLNGLY
17   SERTYRVALVALASN

Samples:

sample_1: PFV RNase H, [U-13C; U-15N], 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
GB ACD84902 ACD84909

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts