BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17798

Title: 1H Chemical Shift Assignments for chicken AvBD2-K31A mutant   PubMed: 22205704

Deposition date: 2011-07-21 Original release date: 2012-04-23

Authors: Meudal, Herve; Landon, Celine

Citation: Derache, Chrystelle; Meudal, Herve; Aucagne, Vincent; Mark, Kevin; Cadene, Martine; Delmas, Agnes; Lalmanach, Anne-Christine; Landon, Celine. "Initial insights into structure-activity relationships of avian defensins"  J. Biol. Chem. 287, 7746-7755 (2012).

Assembly members:
entity, polymer, 36 residues, 3869.558 Da.

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: LFCKGGSCHFGGCPSHLIKV GSCFGFRSCCAWPWNA

Data sets:
Data typeCount
1H chemical shifts226

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1chicken_AvBD2-K31A_mutant1

Entities:

Entity 1, chicken_AvBD2-K31A_mutant 36 residues - 3869.558 Da.

1   LEUPHECYSLYSGLYGLYSERCYSHISPHE
2   GLYGLYCYSPROSERHISLEUILELYSVAL
3   GLYSERCYSPHEGLYPHEARGSERCYSCYS
4   ALATRPPROTRPASNALA

Samples:

sample_1: chicken AvBD2-K31A mutant 2.4 mM; H2O 90%; D2O 10%

sample_2: chicken AvBD2-K31A mutant 2.4 mM; D2O 100%

sample_conditions_1: ionic strength: 0 M; pH: 4.1; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

ARIA v1.1, Linge, O, . - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 17797
PDB
GB AAB30585 AAC36052 AAT48926 AAT76973 ABG73367
REF NP_001188328 NP_990323
SP P46158