BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17834

Title: Syrian hamster prion protein with thiamine   PubMed: 21848803

Deposition date: 2011-08-05 Original release date: 2011-09-13

Authors: Perez-Pineiro, Rolando; Bjorndahl, Trent; Berjanskii, Mark; Hau, David; Li, Li; Huang, Alan; Lee, Rose; Gibbs, Ebrima; Ladner, Carol; Wei Dong, Ying; Abera, Ashenafi; Cashman, Neil; Wishart, David

Citation: Perez-Pineiro, Rolando; Bjorndahl, Trent; Berjanskii, Mark; Hau, David; Li, Li; Huang, Alan; Lee, Rose; Gibbs, Ebrima; Ladner, Carol; Dong, Ying Wei; Abera, Ashenafi; Cashman, Neil; Wishart, David. "The Prion Protein Binds Thiamine"  FEBS J. 278, 4002-4014 (2011).

Assembly members:
shPrP, polymer, 104 residues, 12457.871 Da.
VB1, non-polymer, 349.403 Da.

Natural source:   Common Name: Golden Hamster   Taxonomy ID: 10036   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mesocricetus auratus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
shPrP: LGGYMLGSAMSRPMMHFGND WEDRYYRENMNRYPNQVYYR PVDQYNNQNNFVHDCVNITI KQHTVTTTTKGENFTETDIK IMERVVEQMCTTQYQKESQA YYDG

Data sets:
Data typeCount
15N chemical shifts101
1H chemical shifts183

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1shPrP1
2Thiamine2

Entities:

Entity 1, shPrP 104 residues - 12457.871 Da.

1   LEUGLYGLYTYRMETLEUGLYSERALAMET
2   SERARGPROMETMETHISPHEGLYASNASP
3   TRPGLUASPARGTYRTYRARGGLUASNMET
4   ASNARGTYRPROASNGLNVALTYRTYRARG
5   PROVALASPGLNTYRASNASNGLNASNASN
6   PHEVALHISASPCYSVALASNILETHRILE
7   LYSGLNHISTHRVALTHRTHRTHRTHRLYS
8   GLYGLUASNPHETHRGLUTHRASPILELYS
9   ILEMETGLUARGVALVALGLUGLNMETCYS
10   THRTHRGLNTYRGLNLYSGLUSERGLNALA
11   TYRTYRASPGLY

Entity 2, Thiamine - C13 H23 N3 O6 S - 349.403 Da.

1   VB1

Samples:

sample_1: shPrP, [U-100% 15N], 0.3 mM; thiamine 10 mM; KH2PO4 20 mM; H2O 90%; D2O 10%

sample_2: shPrP 0.5 mM; thiamine 12.5 mM; H2O 90%; D2O 10%

sample_3: shPrP 0.5 mM; thiamine 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
1D STD-TOCSYsample_2isotropicsample_conditions_1
2D NOESYsample_3isotropicsample_conditions_1

Software:

NMRDraw v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 17034 4307
PDB
GB AAA37090 AAA37091 AAA37092 AAA37093 AAB25731
REF XP_005068717 XP_012967855
SP P04273

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts