BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17988

Title: Evolutionary diversification of Mesobuthus {alpha}-scorpion toxins affecting sodium channels.   PubMed: 21969612

Deposition date: 2011-10-09 Original release date: 2011-11-17

Authors: Zhu, Shunyi; Peigneur, Steve; Gao, Bin; Lu, Xiuxiu; Cao, Chunyang; Tytgat, Jan

Citation: Zhu, Shunyi; Peigneur, Steve; Gao, Bin; Lu, Xiuxiu; Cao, Chunyang; Tytgat, Jan. "Evolutionary Diversification of Mesobuthus -Scorpion Toxins Affecting Sodium Channels."  Mol. Cell Proteomics 11, .-. (2012).

Assembly members:
entity, polymer, 66 residues, 7187.264 Da.

Natural source:   Common Name: scorpions   Taxonomy ID: 34646   Superkingdom: Metazoa   Kingdom: Mesobuthus   Genus/species: eupeus not available

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
entity: ARDAYIAKPHNCVYECFDAF SSYCNGVCTKNGAKSGYCQI LGTYGNGCWCIALPDNVPIR IPGKCH

Data sets:
Data typeCount
1H chemical shifts422

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mesobuthus {alpha}-scorpion toxins affecting sodium channels1

Entities:

Entity 1, Mesobuthus {alpha}-scorpion toxins affecting sodium channels 66 residues - 7187.264 Da.

1   ALAARGASPALATYRILEALALYSPROHIS
2   ASNCYSVALTYRGLUCYSPHEASPALAPHE
3   SERSERTYRCYSASNGLYVALCYSTHRLYS
4   ASNGLYALALYSSERGLYTYRCYSGLNILE
5   LEUGLYTHRTYRGLYASNGLYCYSTRPCYS
6   ILEALALEUPROASPASNVALPROILEARG
7   ILEPROGLYLYSCYSHIS

Samples:

sample_1: sodium phosphate 50 mM; H2O 90%; D2O 90%; entity 1 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 4.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Brunger - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB ABR21068 ADF49571 ADF49572 ADF49573 ADT82852
SP P86404 P86405