BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18009

Title: NMR structure of the lambda 5 22-45 peptide   PubMed: 23124203

Deposition date: 2011-10-19 Original release date: 2012-10-22

Authors: Elantak, Latifa; Espeli, Marion; Boned, Annie; Bornet, Olivier; Breton, Caroline; Feracci, Mikael; Roche, Philippe; Guerlesquin, Francoise; Schiff, Claudine

Citation: Elantak, Latifa; Espeli, Marion; Boned, Annie; Bornet, Olivier; Bonzi, Jeremy; Gauthier, Laurent; Feracci, Mikael; Roche, Philippe; Guerlesquin, Francoise; Schiff, Claudine. "Structural basis for galectin-1-dependent pre-B cell receptor (pre-BCR) activation."  J. Biol. Chem. 287, 44703-44713 (2012).

Assembly members:
entity, polymer, 24 residues, 2856.299 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: SRSSLRSRWGRFLLQRGSWT GPRC

Data sets:
Data typeCount
1H chemical shifts159

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1lambda_5_22-45_peptide1

Entities:

Entity 1, lambda_5_22-45_peptide 24 residues - 2856.299 Da.

1   SERARGSERSERLEUARGSERARGTRPGLY
2   ARGPHELEULEUGLNARGGLYSERTRPTHR
3   GLYPROARGCYS

Samples:

sample_1: potassium phosphate 20 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.2; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAI46548
GB AAA36096 AAA36100 AAH12293 ABM84504 ABM85566
REF NP_064455 XP_003282108 XP_003814324 XP_004063189 XP_004063194
SP P15814