BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18111

Title: Structure of T7 transcription factor Gp2-E. coli RNAp jaw domain complex

Deposition date: 2011-11-29 Original release date: 2012-01-25

Authors: Liu, Minhao

Citation: Liu, Minhao; Matthews, Stephen; James, Ellen; Sheppard, Carol. "Structure of T7 transcription factor Gp2-E. coli RNAp jaw domain complex"  Mol. Cell ., .-..

Assembly members:
entity_1, polymer, 84 residues, 6635.446 Da.
entity_2, polymer, 84 residues, 6795.774 Da.

Natural source:   Common Name: not available   Taxonomy ID: 10760   Superkingdom: not available   Kingdom: not available   Genus/species: Bacteriophage T7

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MSNVNTGSLSVDNKKFWATV ESSEHSFEVPIYAETLDEAL ELAEWQYVPAGFEVTRVRPC VAPK
entity_2: MGSSHHHHHHSSGLVPRGSH MKEPAILAEISGIVSFGKET KGKRRLVITPVDGSDPYEEM IPKWRQLNVFEGERVERGDV ISDG

Data sets:
Data typeCount
13C chemical shifts499
15N chemical shifts113
1H chemical shifts762

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 84 residues - 6635.446 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METSERASNVALASNTHRGLYSERLEUSER
4   VALASPASNLYSLYSPHETRPALATHRVAL
5   GLUSERSERGLUHISSERPHEGLUVALPRO
6   ILETYRALAGLUTHRLEUASPGLUALALEU
7   GLULEUALAGLUTRPGLNTYRVALPROALA
8   GLYPHEGLUVALTHRARGVALARGPROCYS
9   VALALAPROLYS

Entity 2, entity_2 84 residues - 6795.774 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METLYSGLUPROALAILELEUALAGLUILE
4   SERGLYILEVALSERPHEGLYLYSGLUTHR
5   LYSGLYLYSARGARGLEUVALILETHRPRO
6   VALASPGLYSERASPPROTYRGLUGLUMET
7   ILEPROLYSTRPARGGLNLEUASNVALPHE
8   GLUGLYGLUARGVALGLUARGGLYASPVAL
9   ILESERASPGLY

Samples:

sample_1: Gp2-Jaw complex, [U-100% 13C; U-100% 15N], 300 mM; Gp2-Jaw complex, [U-100% 13C; U-100% 15N], 300 mM; H2O 90%; D2O 10%; NaCl 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DPX 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts