BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18146

Title: Insight into the antimicrobial activities based on the Structure-activity relationships of coprisin isolated from the Dung Beetle, Copris tripartitus   PubMed: 23137439

Deposition date: 2011-12-16 Original release date: 2012-11-15

Authors: Kim, Yangmee; Kim, Jin-Kyoung; Lee, Eunjung

Citation: Lee, Eunjung; Kim, Jin-Kyoung; Shin, Soyoung; Jeong, Ki-Woong; Shin, Areum; Lee, Juneyoung; Lee, Dong Gun; Hwang, Jae-Sam; Kim, Yangmee. "Insight into the antimicrobial activities of coprisin isolated from the dung beetle, Copris tripartitus, revealed by structure-activity relationships."  Biochim. Biophys. Acta 1828, 271-283 (2013).

Assembly members:
entity, polymer, 44 residues, 4482.301 Da.

Natural source:   Common Name: Beetle   Taxonomy ID: 438892   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Copris tripartitus

Experimental source:   Production method: chemical synthesis   Host organism: synthetic peptide

Entity Sequences (FASTA):
entity: VTCDVLSFEAKGIAVNHSAC ALHCIALRKKGGSCQNGVCV CRNX

Data sets:
Data typeCount
1H chemical shifts271

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1coprisin1

Entities:

Entity 1, coprisin 44 residues - 4482.301 Da.

1   VALTHRCYSASPVALLEUSERPHEGLUALA
2   LYSGLYILEALAVALASNHISSERALACYS
3   ALALEUHISCYSILEALALEUARGLYSLYS
4   GLYGLYSERCYSGLNASNGLYVALCYSVAL
5   CYSARGASNNH2

Samples:

sample_1: entity 1 mM; H2O 90%; D2O 10%

conditions_1: ionic strength: 100 mM; pH: 4.3; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicconditions_1
2D 1H-1H TOCSYsample_1isotropicconditions_1
2D DQF-COSYsample_1isotropicconditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
GB ABP97087