BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18335

Title: Structure and orientation of the gH625-644 membrane interacting region of herpes simplex virus type 1 in a membrane mimetic system.   PubMed: 22397737

Deposition date: 2012-03-19 Original release date: 2012-04-16

Authors: Isernia, Carla; Galdiero, Stefania; Russo, Luigi; Falanga, Annarita; Cantisani, Marco; Vitiello, MariaTeresa; Fattorusso, Roberto; Malgieri, Gaetano; Galdiero, Massimiliano

Citation: Galdiero, Stefania; Russo, Luigi; Falanga, Annarita; Cantisani, Marco; Vitiello, Mariateresa; Fattorusso, Roberto; Malgieri, Gaetano; Galdiero, Massimiliano; Isernia, Carla. "Structure and orientation of the gH625-644 membrane interacting region of herpes simplex virus type 1 in a membrane mimetic system."  Biochemistry 51, 3121-3128 (2012).

Assembly members:
entity_1, polymer, 20 residues, 2302.661 Da.
ACETYL GROUP, non-polymer, 44.053 Da.
N'-{4-[2-(DIAMINOMETHYLIDENE)HYDRAZONO]CYCLOHEXYLIDEN}AMINOMETHANEHYDRAZONAMIDE, non-polymer, 224.266 Da.

Natural source:   Common Name: HSV-1   Taxonomy ID: 10298   Superkingdom: Viruses   Kingdom: not available   Genus/species: Simplexvirus not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: HGLASTLTRWAHYNALIRAF

Data sets:
Data typeCount
1H chemical shifts105

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gH625-6441
2ACETYL GROUP2
3N'-{4-[2-(DIAMINOMETHYLIDENE)HYDRAZONO]CYCLOHEXYLIDEN}AMINOMETHANEHYDRAZONAMIDE3

Entities:

Entity 1, gH625-644 20 residues - 2302.661 Da.

1   HISGLYLEUALASERTHRLEUTHRARGTRP
2   ALAHISTYRASNALALEUILEARGALAPHE

Entity 2, ACETYL GROUP - C2 H4 O - 44.053 Da.

1   ACE

Entity 3, N'-{4-[2-(DIAMINOMETHYLIDENE)HYDRAZONO]CYCLOHEXYLIDEN}AMINOMETHANEHYDRAZONAMIDE - C8 H16 N8 - 224.266 Da.

1   NHH

Samples:

sample_1: gH625-644 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

BMRB 15647 16699
PDB
DBJ BAM73371
EMBL CAA27534 CAA32335
GB AAA45815 AAG17895 ABI63484 ACM62244 ADD59995
REF YP_009137096
SP P06477 P08356 Q9DHD5