BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18536

Title: KB1[GHRW;23-28]   PubMed: 23012369

Deposition date: 2012-06-20 Original release date: 2012-08-28

Authors: Daly, Norelle; Eliasen, Rasmus; Craik, David

Citation: Eliasen, Rasmus; Daly, Norelle; Wulff, Birgitte; Andresen, Thomas; Conde-Frieboes, Kilian; Craik, David. "Design, synthesis, structural and functional characterization of novel melanocortin agonists based on the cyclotide kalata B1."  J. Biol. Chem. 287, 40493-40501 (2012).

Assembly members:
entity, polymer, 29 residues, 3020.448 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: CGETCVGGTCNTPGCTCSWP VCGHFRWGV

Data sets:
Data typeCount
1H chemical shifts156

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KB1[GHRW; 23-28]1

Entities:

Entity 1, KB1[GHRW; 23-28] 29 residues - 3020.448 Da.

1   CYSGLYGLUTHRCYSVALGLYGLYTHRCYS
2   ASNTHRPROGLYCYSTHRCYSSERTRPPRO
3   VALCYSGLYHISPHEARGTRPGLYVAL

Samples:

sample_1: entity 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

CcpNMR, University of Cambridge - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 16235 18506 19611
PDB
GB AAL05477 ACI29319 ADJ17473
PIR A56283
SP P56254 P83938 P85185