BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18568

Title: ns4b40

Deposition date: 2012-07-05 Original release date: 2013-06-17

Authors: Montserret, Roland; Penin, Francois

Citation: Montserret, Roland; Penin, Francois. "Aminoterminal Amphipathic alpha-Helix AH1 of Hepatitis C Virus Nonstructural Protein 4B Possesses a Dual Role in RNA Replication and Virus Production"  PLOS Pathogens ., .-..

Assembly members:
entity, polymer, 40 residues, 4385.087 Da.

Natural source:   Common Name: Hepatitis C Virus   Taxonomy ID: 11103   Superkingdom: Viruses   Kingdom: not available   Genus/species: Hepacivirus Hepatitis C virus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: ASRAALIEEGQRIAEMLKSK IQGLLQQASKQAQDIQPAMQ

Data sets:
Data typeCount
13C chemical shifts132
1H chemical shifts293

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NS4B1

Entities:

Entity 1, NS4B 40 residues - 4385.087 Da.

1   ALASERARGALAALALEUILEGLUGLUGLY
2   GLNARGILEALAGLUMETLEULYSSERLYS
3   ILEGLNGLYLEULEUGLNGLNALASERLYS
4   GLNALAGLNASPILEGLNPROALAMETGLN

Samples:

sample_1: H2O 40 v/v; D2O, [U-100% 2H], 10 v/v; TFE, [U-100% 2H], 50 v/v; DSS 0.2 uM; NS4B 1.2 mM

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ BAA00792 BAB32872 BAB32874 BAB32875 BAD06942
GB AAC53944 AAC53945 AAF25610 AAF25613 ABX82715
SP P26660 Q99IB8