BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18877

Title: 1H,13C,15N chemical shift assignment of Ca2+_bound CaBP4   PubMed: 23925854

Deposition date: 2012-12-05 Original release date: 2014-02-14

Authors: Ames, James

Citation: Park, Saebomi; Li, Congmin; Ames, James. "(1)H, (15)N, and (13)C chemical shift assignments of murine calcium-binding protein 4."  Biomol. NMR Assignments ., .-. (2013).

Assembly members:
CaBP4, polymer, 271 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CaBP4: MATEHNVQLVPGSQKIPKGV VSPRSAAEGPALTRRRSKKE SWHPGSQKASSGDQSSSQGS EASGSSKHPPRTKVGQEEPS SAPARPASHRHSHRHRSDPQ QDAAQRTYGPLLNRMFGKDR ELGPEELEELQAAFEEFDTD QDGYIGYRELGDCMRTLGYM PTEMELLEVSQHVKMRMGGF VDFEEFVELISPKLREETAH MLGVRELRIAFREFDKDRDG RITVAELRQAAPALLGEPLE GTELDEMLREMDLNGDGTID FDEFVMMLSTG

Data sets:
Data typeCount
13C chemical shifts542
15N chemical shifts159
1H chemical shifts761

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CaBP4 polypeptide1

Entities:

Entity 1, CaBP4 polypeptide 271 residues - Formula weight is not available

1   METALATHRGLUHISASNVALGLNLEUVAL
2   PROGLYSERGLNLYSILEPROLYSGLYVAL
3   VALSERPROARGSERALAALAGLUGLYPRO
4   ALALEUTHRARGARGARGSERLYSLYSGLU
5   SERTRPHISPROGLYSERGLNLYSALASER
6   SERGLYASPGLNSERSERSERGLNGLYSER
7   GLUALASERGLYSERSERLYSHISPROPRO
8   ARGTHRLYSVALGLYGLNGLUGLUPROSER
9   SERALAPROALAARGPROALASERHISARG
10   HISSERHISARGHISARGSERASPPROGLN
11   GLNASPALAALAGLNARGTHRTYRGLYPRO
12   LEULEUASNARGMETPHEGLYLYSASPARG
13   GLULEUGLYPROGLUGLULEUGLUGLULEU
14   GLNALAALAPHEGLUGLUPHEASPTHRASP
15   GLNASPGLYTYRILEGLYTYRARGGLULEU
16   GLYASPCYSMETARGTHRLEUGLYTYRMET
17   PROTHRGLUMETGLULEULEUGLUVALSER
18   GLNHISVALLYSMETARGMETGLYGLYPHE
19   VALASPPHEGLUGLUPHEVALGLULEUILE
20   SERPROLYSLEUARGGLUGLUTHRALAHIS
21   METLEUGLYVALARGGLULEUARGILEALA
22   PHEARGGLUPHEASPLYSASPARGASPGLY
23   ARGILETHRVALALAGLULEUARGGLNALA
24   ALAPROALALEULEUGLYGLUPROLEUGLU
25   GLYTHRGLULEUASPGLUMETLEUARGGLU
26   METASPLEUASNGLYASPGLYTHRILEASP
27   PHEASPGLUPHEVALMETMETLEUSERTHR
28   GLY

Samples:

sample_1: CaBP4, [U-13C; U-15N; U-2H], 0.5 mM

sample_2: CaBP4, [U-15N], 0.5 mM

sample_3: CaBP4, [U-13C; U-15N], 0.5 mM

sample_4: CaBP4 0.5 mM

sample_5: CaBP4, [U-10% 13C; U-99% 15N], 0.5 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 310 K

sample_conditions_2: pH: 7.4; pressure: 1 atm; temperature: 310 K

sample_conditions_3: pH: 7.4; pressure: 1 atm; temperature: 310 K

sample_conditions_4: pH: 7.4; pressure: 1 atm; temperature: 310 K

sample_conditions_5: pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
3D HNCOsample_3isotropicsample_conditions_3
3D HN(CA)COsample_3isotropicsample_conditions_3
Trosy HNCACBsample_1isotropicsample_conditions_1
Trosy HN(CO)CACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_2
2D 1H-1H COSYsample_4isotropicsample_conditions_4
2D 1H-1H NOESYsample_4isotropicsample_conditions_4
13C CT-HSQCsample_5isotropicsample_conditions_5

Software:

SPARKY, Goddard - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAH49263 AAK83463 EDL33015 EDL33016
REF NP_653115 XP_006531910 XP_006531911 XP_006531912 XP_006544976
SP Q8VHC5

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts