BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19094

Title: Enterocin 7A   PubMed: 23725536

Deposition date: 2013-03-16 Original release date: 2013-04-16

Authors: Lohans, Christopher; Towle, Kaitlyn; Miskolzie, Mark; McKay, Ryan; van Belkum, Marco; McMullen, Lynn; Vederas, John

Citation: Lohans, Christopher; Towle, Kaitlyn; Miskolzie, Mark; McKay, Ryan; van Belkum, Marco; McMullen, Lynn; Vederas, John. "Solution Structures of the Linear Leaderless Bacteriocins Enterocin 7A and 7B Resemble Carnocyclin A, a Circular Antimicrobial Peptide"  Biochemistry 52, 3987-3994 (2013).

Assembly members:
Enterocin_7A, polymer, 44 residues, 5213.414 Da.

Natural source:   Common Name: Firmicutes   Taxonomy ID: 1351   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Enterococcus faecalis

Experimental source:   Production method: purified from the natural source   Host organism: Enterococcus faecalis

Entity Sequences (FASTA):
Enterocin_7A: XGAIAKLVAKFGWPIVKKYY KQIMQFIGEGWAINKIIDWI KKHI

Data sets:
Data typeCount
1H chemical shifts316

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Enterocin 7A1

Entities:

Entity 1, Enterocin 7A 44 residues - 5213.414 Da.

1   FMEGLYALAILEALALYSLEUVALALALYS
2   PHEGLYTRPPROILEVALLYSLYSTYRTYR
3   LYSGLNILEMETGLNPHEILEGLYGLUGLY
4   TRPALAILEASNLYSILEILEASPTRPILE
5   LYSLYSHISILE

Samples:

sample_1: Enterocin 7A 0.8 mM; DSS 0.01 w/v; H2O 90%; D2O 10%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Varian VNMRS 700 MHz

Related Database Links:

PDB
DBJ BAF74788
EMBL CAA11520
GB ABB46250 ABC95646 ABL11217 ACM68960 AFU07735
REF WP_002293184 YP_001966103 YP_009082434