BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19179

Title: ORF PP_3909 from Pseudomonas putida KT2440 encoding a protein similar to bacteriophage lambda ea8.5   PubMed: 23672713

Deposition date: 2013-04-18 Original release date: 2013-06-10

Authors: Donaldson, Logan

Citation: Kwan, Jamie; Smirnova, Ekaterina; Khazai, Shahin; Evanics, Ferenc; Maxwell, Karen; Donaldson, Logan. "The Solution Structures of Two Prophage Homologues of the Bacteriophage Ea8.5 Protein Reveal a Newly Discovered Hybrid Homeodomain/Zinc-Finger Fold."  Biochemistry 52, 3612-3614 (2013).

Assembly members:
mol1, polymer, 92 residues, 10196.0386 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 303   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas putida

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
mol1: MGGIKRLMEEEDAKYSEAVY IAIEAGTLAECEVHEGTYFS DSGDISEAEELAREKFEKGE VSNFDDVEELVKKVVAVCEE LGAEECFSCDFD

Data sets:
Data typeCount
13C chemical shifts307
15N chemical shifts75
1H chemical shifts477

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1mol11
2Zinc ions2

Entities:

Entity 1, mol1 92 residues - 10196.0386 Da.

1   METGLYGLYILELYSARGLEUMETGLUGLU
2   GLUASPALALYSTYRSERGLUALAVALTYR
3   ILEALAILEGLUALAGLYTHRLEUALAGLU
4   CYSGLUVALHISGLUGLYTHRTYRPHESER
5   ASPSERGLYASPILESERGLUALAGLUGLU
6   LEUALAARGGLULYSPHEGLULYSGLYGLU
7   VALSERASNPHEASPASPVALGLUGLULEU
8   VALLYSLYSVALVALALAVALCYSGLUGLU
9   LEUGLYALAGLUGLUCYSPHESERCYSASP
10   PHEASP

Entity 2, Zinc ions - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: mol1, [U-99% 13C; U-99% 15N], 0.6 mM; H20 90%; D20 10%

condition_1: ionic strength: 0.150 M; pH: 7.800; pressure: 1.000 atm; temperature: 293.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCsample_1isotropiccondition_1
2D 1H-13C HSQC aliphaticsample_1isotropiccondition_1
2D 1H-13C HSQC aromaticsample_1isotropiccondition_1
3D CBCA(CO)NHsample_1isotropiccondition_1
3D C(CO)NHsample_1isotropiccondition_1
3D HNCOsample_1isotropiccondition_1
3D HNCACBsample_1isotropiccondition_1
3D HBHA(CO)NHsample_1isotropiccondition_1
3D HN(CA)COsample_1isotropiccondition_1
3D H(CCO)NHsample_1isotropiccondition_1
2D CBHDsample_1isotropiccondition_1
2D CBHEsample_1isotropiccondition_1
3D (H)C(CO)NHsample_1isotropiccondition_1
3D 1H-15N NOESYsample_1isotropiccondition_1
3D 1H-13C NOESY aliphaticsample_1isotropiccondition_1
3D 1H-13C NOESY aromaticsample_1isotropiccondition_1

Software:

ANALYSIS v2.2, CCPN - data analysis

CYANA v3, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian NMRS 600 MHz

Related Database Links:

GenBank 24987239
PDB
GB AAN69503 KMU94681 KMY31145
REF NP_746039 WP_010954735

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts