BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19275

Title: Solution Structure and Activity Study of Bovicin HJ50, a Particular Type AII Lantibiotic   PubMed: 24814218

Deposition date: 2013-05-29 Original release date: 2014-05-20

Authors: Zhang, Jie; Feng, Yingang; Wang, Jinfeng; Zhong, Jin

Citation: Zhang, Jie; Feng, Yingang; Wang, Jinfeng; Zhong, Jin. "Type AII Lantibiotic Bovicin HJ50 with a Rare Disulfide Bond: Structure, Structure-Activity Relationships and Mode of Action"  Biochem. J. ., .-. (2014).

Assembly members:
entity_1, polymer, 33 residues, 3156.733 Da.

Natural source:   Common Name: Streptococcus equinus   Taxonomy ID: 1335   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus bovis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: ADRGWIKXLXKDCPNVISSI CAGTIITACKNCA

Data sets:
Data typeCount
13C chemical shifts106
1H chemical shifts219

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Bovicin HJ501

Entities:

Entity 1, Bovicin HJ50 33 residues - 3156.733 Da.

1   ALAASPARGGLYTRPILELYSDBBLEUDBB
2   LYSASPCYSPROASNVALILESERSERILE
3   CYSALAGLYTHRILEILETHRALACYSLYS
4   ASNCYSALA

Samples:

sample_1: entity_1 1 mM; sodium acetate, [U-2H], 50 mM; DSS 0.01%

sample_2: entity_1 1 mM; sodium acetate, [U-2H], 50 mM; DSS 0.01%

sample_conditions_1: ionic strength: 50 mM; pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D DQF-COSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

FELIX, Accelrys Software Inc. - processing

NMRViewJ, Johnson, One Moon Scientific - chemical shift assignment

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

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