BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19304

Title: Solution structure of kalata B7   PubMed: 23893463

Deposition date: 2013-06-18 Original release date: 2013-11-18

Authors: Daly, Norelle; Elliott, Alysha; Craik, David

Citation: Rosengren, K. Johan; Daly, Norelle; Harvey, Peta; Craik, David. "The self-association of the cyclotide kalata B2 in solution is guided by hydrophobic interactions."  Biopolymers 100, 453-460 (2013).

Assembly members:
entity, polymer, 29 residues, 3097.613 Da.

Natural source:   Common Name: Oldenlandia affinis   Taxonomy ID: 60225   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Oldenlandia affinis

Experimental source:   Production method: purified from the natural source   Host organism: Oldenlandia affinis

Entity Sequences (FASTA):
entity: GLPVCGETCTLGTCYTQGCT CSWPICKRN

Data sets:
Data typeCount
1H chemical shifts182

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1kalata B71

Entities:

Entity 1, kalata B7 29 residues - 3097.613 Da.

1   GLYLEUPROVALCYSGLYGLUTHRCYSTHR
2   LEUGLYTHRCYSTYRTHRGLNGLYCYSTHR
3   CYSSERTRPPROILECYSLYSARGASN

Samples:

sample_1: entity 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 4; pressure: 1 atm; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB AAL05479 ACI29320
SP P58457