BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19525

Title: Solution structure of the Nt. GR-RBP1 RRM domain

Deposition date: 2013-09-24 Original release date: 2013-09-30

Authors: Kahn, F.; Boelens, R.; Saqlan, S.; van Ingen, Hugo

Citation: Kahn, F.; Boelens, R.; Saqlan, S.; van Ingen, Hugo. "Structural basis of nucleic acid binding by Nicotiana tabacum glycine-rich RNA binding protein: implications for its RNA chaperone function"  To be published ., .-..

Assembly members:
RNA-BINDING_GLYCINE-RICH_PROTEIN, polymer, 86 residues, 9522.4633 Da.

Natural source:   Common Name: Tobacco   Taxonomy ID: 4097   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Nicotiana Tabacum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RNA-BINDING_GLYCINE-RICH_PROTEIN: GMAEVEYRCFVGGLAWATTD QTLGEAFSQFGEILDSKIIN DRETGRSRGFGFVTFKDEKA MRDAIEGMNGQDLDGRNITV NEAQSR

Data sets:
Data typeCount
13C chemical shifts362
15N chemical shifts93
1H chemical shifts563

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA-BINDING GLYCINE-RICH PROTEIN1

Entities:

Entity 1, RNA-BINDING GLYCINE-RICH PROTEIN 86 residues - 9522.4633 Da.

1   GLYMETALAGLUVALGLUTYRARGCYSPHE
2   VALGLYGLYLEUALATRPALATHRTHRASP
3   GLNTHRLEUGLYGLUALAPHESERGLNPHE
4   GLYGLUILELEUASPSERLYSILEILEASN
5   ASPARGGLUTHRGLYARGSERARGGLYPHE
6   GLYPHEVALTHRPHELYSASPGLULYSALA
7   METARGASPALAILEGLUGLYMETASNGLY
8   GLNASPLEUASPGLYARGASNILETHRVAL
9   ASNGLUALAGLNSERARG

Samples:

sample_1: RNA-BINDING GLYCINE-RICH PROTEIN, [U-13C; U-15N], 0.4 mM

sample_conditions_1: ionic strength: 150.000 mM; pH: 7.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1solutionsample_conditions_1

Software:

AutoDep v4.3, PDBe - chemical shift assignment

ANALYSIS vany, CCPN - chemical shift assignment

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 750 MHz

Related Database Links:

GB ADG03637.1 ABH07505 ADG03637
UNP D6PZY5_TOBAC
PDB
DBJ BAA03741
REF XP_009774643

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts