BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19675

Title: Solution structure of the CLAVATA encoded peptide of Arabidopsis thaliana - AtCLE44

Deposition date: 2013-12-13 Original release date: 2014-12-22

Authors: Bobay, Benjamin; DiGennaro, Peter; Bird, David

Citation: DiGennaro, Peter; Bobay, Benjamin; Bird, David. "Inferring function of CLE peptides from high resolution tertiary structures"  Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:
entity, polymer, 12 residues, 1248.334 Da.

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: HEVPSGPNPISN

Data sets:
Data typeCount
13C chemical shifts29
15N chemical shifts10
1H chemical shifts69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CLAVATA encoded peptide of Arabidopsis thaliana - AtCLE441

Entities:

Entity 1, CLAVATA encoded peptide of Arabidopsis thaliana - AtCLE44 12 residues - 1248.334 Da.

1   HISGLUVALPROSERGLYPROASNPROILE
2   SERASN

Samples:

sample_1: entity 4 mg/mL; potassium chloride 2.7 mM; potassium phosphate 1.8 mM; sodium chloride 137 mM; sodium phosphate 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 140 mM; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

ARIA, Linge, O, . - refinement, structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts