BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19854

Title: Structural Characterization of the Mengovirus Leader Protein Bound to Ran GTPase by Nuclear Magnetic Resonance

Deposition date: 2014-03-15 Original release date: 2014-10-07

Authors: Bacot-Davis, Valjean; Palmenberg, Ann; Cornilescu, Claudia; Markley, John

Citation: Bacot-Davis, Valjean; Ciomperlik, J.; Basta, H.; Cornilescu, Claudia; Palmenberg, Ann. "Solution Structures of Mengovirus Leader Protein, its Phosphorylated Derivatives, and in Complex with Nuclear Transport Protein, RanGTPase"  Proc. Nat. Acad. Sci, U.S.A. ., .-. (2014).

Assembly members:
entity, polymer, 216 residues, 24456.295 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: MAAQGEPQVQFKLVLVGDGG TGKTTFVKRHLTGEFEKKYV ATLGVEVHPLVFHTNRGPIK FNVWDTAGQEKFGGLRDGYY IQAQCAIIMFDVTSRVTYKN VPNWHRDLVRVCENIPIVLC GNKVDIKDRKVKAKSIVFHR KKNLQYYDISAKSNYNFEKP FLWLARKLIGDPNLEFVAMP ALAPPEVVMDPALAAQYEHD LEVAQTTALPDEDDDL

Data sets:
Data typeCount
13C chemical shifts899
15N chemical shifts294
1H chemical shifts1438

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 216 residues - 24456.295 Da.

1   METALAALAGLNGLYGLUPROGLNVALGLN
2   PHELYSLEUVALLEUVALGLYASPGLYGLY
3   THRGLYLYSTHRTHRPHEVALLYSARGHIS
4   LEUTHRGLYGLUPHEGLULYSLYSTYRVAL
5   ALATHRLEUGLYVALGLUVALHISPROLEU
6   VALPHEHISTHRASNARGGLYPROILELYS
7   PHEASNVALTRPASPTHRALAGLYGLNGLU
8   LYSPHEGLYGLYLEUARGASPGLYTYRTYR
9   ILEGLNALAGLNCYSALAILEILEMETPHE
10   ASPVALTHRSERARGVALTHRTYRLYSASN
11   VALPROASNTRPHISARGASPLEUVALARG
12   VALCYSGLUASNILEPROILEVALLEUCYS
13   GLYASNLYSVALASPILELYSASPARGLYS
14   VALLYSALALYSSERILEVALPHEHISARG
15   LYSLYSASNLEUGLNTYRTYRASPILESER
16   ALALYSSERASNTYRASNPHEGLULYSPRO
17   PHELEUTRPLEUALAARGLYSLEUILEGLY
18   ASPPROASNLEUGLUPHEVALALAMETPRO
19   ALALEUALAPROPROGLUVALVALMETASP
20   PROALALEUALAALAGLNTYRGLUHISASP
21   LEUGLUVALALAGLNTHRTHRALALEUPRO
22   ASPGLUASPASPASPLEU

Samples:

sample_1: RAN GTPASE, [U-100% 13C; U-100% 15N], 0.5 mM; L MENGO 0.5 mM; HEPES 20 mM; potassium chloride 100 mM; magnesium chloride 2 mM; DTT 2 mM; sodium azide 0.04%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 102 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
31-Psample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

SPARTA+, Shen and Bax - geometry optimization

SPARKY, Goddard - peak picking

CARA, Kurt W. Thrich, Swiss Federal Institute of Technology ETH - peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 19852
PDB
DBJ BAA89696 BAB27034 BAB27105 BAB93486 BAC36040
EMBL CAA10191 CAA47355 CAA77980 CAG29343 CAH92646
GB AAA36546 AAA64247 AAB24940 AAB50841 AAC05840
PRF 1814339A
REF NP_001004829 NP_001029877 NP_001080182 NP_001126549 NP_001128547
SP P42558 P52301 P62825 P62826 P62827
TPG DAA20795

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts