BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19960

Title: Solution structure of the human chemokine CCL19

Deposition date: 2014-05-09 Original release date: 2015-06-08

Authors: Veldkamp, C.; Peterson, F.; Gabel-Eissens, S.; Gillitzer, M.

Citation: Veldkamp, C.; Peterson, F.; Gabel-Eissens, S.; Gillitzer, M.. "Solution structure of the human chemokine CCL19"  To be published ., .-..

Assembly members:
Human_Chemokine_CCL19, polymer, 77 residues, 8814.347 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Human_Chemokine_CCL19: GTNDAEDCCLSVTQKPIPGY IVRNFHYLLIKDGCRVPAVV FTTLRGRQLCAPPDQPWVER IIQRLQRTSAKMKRRSS

Data sets:
Data typeCount
13C chemical shifts267
15N chemical shifts77
1H chemical shifts557

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human Chemokine CCL191

Entities:

Entity 1, Human Chemokine CCL19 77 residues - 8814.347 Da.

1   GLYTHRASNASPALAGLUASPCYSCYSLEU
2   SERVALTHRGLNLYSPROILEPROGLYTYR
3   ILEVALARGASNPHEHISTYRLEULEUILE
4   LYSASPGLYCYSARGVALPROALAVALVAL
5   PHETHRTHRLEUARGGLYARGGLNLEUCYS
6   ALAPROPROASPGLNPROTRPVALGLUARG
7   ILEILEGLNARGLEUGLNARGTHRSERALA
8   LYSMETLYSARGARGSERSER

Samples:

sample: Human Chemokine CCL19, [U-100% 13C; U-100% 15N], 0.515 mM; D2O, [U-99% 2H], 10%; dueterated MES, [U-100% 2H], 25 mM

sample_conditions_1: ionic strength: 6 mM; pH: 5.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsampleisotropicsample_conditions_1
3D 13C-separated NOESYsampleisotropicsample_conditions_1
3D 13C-separated NOESY (AROMATIC)sampleisotropicsample_conditions_1

Software:

Xplor-NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement

TopSpin v3.1, Bruker - collection

nmrPipe v2007, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis

GARANT v2.1, C. Bartels - data analysis

CARA v1.8.4, Keller, R. - data analysis

Cyana v3.1, Guntert, P. - structural calculation

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAA20383 BAG34886 BAJ20579
EMBL CAA11307 CAG33149
GB AAC23905 AAC50944 AAH27968 AAS20190 ABK41949
REF NP_006265 XP_001164037 XP_002819711 XP_003829861 XP_004048020
SP Q99731

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts