BMRB Entry 20121
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20121
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Title: Tubulysin conformation bound to tubulin PubMed: 20496362
Deposition date: 2010-02-03 Original release date: 2010-07-26
Authors: Kubicek, Karel; Grimm, Steffen; Orts, Julien; Sasse, Florenz; Carlomagno, Teresa
Citation: Kubicek, Karel; Grimm, S. Kaspar; Orts, Julien; Sasse, Florenz; Carlomagno, Teresa. "The tubulin-bound structure of the antimitotic drug tubulysin." Angew. Chem. Int. Ed. Engl. 49, 4809-4812 (2010).
Assembly members:
tubulysin, polymer, 4 residues, 841 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
tubulysin: XIXX
- assigned_chemical_shifts
- conformer_family_coord_set
Data type | Count |
1H chemical shifts | 56 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | tubulysin | 1 |
Entities:
Entity 1, tubulysin 4 residues - 841 Da.
1 | T23 | ILE | TUV | TUT |
Samples:
sample_1: tubulysin 0.5 mM; tubulin 0.01 mM; sodium phosphate 1.5 mM; calcium phosphate 1.5 mM; H2O 95%; DMSO 5%
sample_conditions_1: temperature: 298 K; pH: 7.0
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR_NIH v2.31, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 900 MHz