BMRB Entry 21006
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR21006
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Title: alpha-conotoxin ImI cystathionine 2-4 PubMed: 21899353
Deposition date: 2011-04-21 Original release date: 2011-10-27
Authors: Dekan, Zoltan; Vetter, Irina; Daly, Norelle; Lewis, Richard; Alewood, Paul; Craik, David
Citation: Dekan, Zoltan; Vetter, Irina; Daly, Norelle; Craik, David; Lewis, Richard; Alewood, Paul. "Alpha-Conotoxin ImI Incorporating Stable Cystathionine Bridges Maintains Full Potency and Identical Three-Dimensional Structure" J. Am. Chem. Soc. 133, 15866-15869 (2011).
Assembly members:
alpha_conotoxin_ImI_Cystathionine_2-4, polymer, 12 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
alpha_conotoxin_ImI_Cystathionine_2-4: GCXSDPRCAWRC
- assigned_chemical_shifts
- conformer_family_coord_set
Data type | Count |
1H chemical shifts | 65 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | alpha_conotoxin_ImI_Cystathionine_2-4 | 1 |
Entities:
Entity 1, alpha_conotoxin_ImI_Cystathionine_2-4 12 residues - Formula weight is not available
X=Cystathionine
1 | GLY | CYS | CTT | SER | ASP | PRO | ARG | CYS | ALA | TRP | ||||
2 | ARG | CYS |
Samples:
sample_1: alpha conotoxin ImI Cystathionine 2-4 1 mM; H2O 90%; D2O 10%
sample_conditions_1: temperature: 280 K; pH: 3
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
SPARKY, Goddard - chemical shift assignment
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz