BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25131

Title: 1H chemical shift assignment of Kindlin-2 F2

Deposition date: 2014-08-05 Original release date: 2014-08-25

Authors: Fukuda, Koichi; Bledzka, Kamila; Yang, Jun; Perera, H.

Citation: Fukuda, Koichi; Bledzka, Kamila; Yang, Jun; Perera, H.; Edward, Plow; Qin, Jun. "kindlin-2 F2 domain"  J. Biol. Chem. ., .-..

Assembly members:
entity, polymer, 20 residues, 2192.330 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: SDKEVDEVDAALSDLEITLE

Data sets:
Data typeCount
1H chemical shifts112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Kindlin-2 F21

Entities:

Entity 1, Kindlin-2 F2 20 residues - 2192.330 Da.

1   SERASPLYSGLUVALASPGLUVALASPALA
2   ALALEUSERASPLEUGLUILETHRLEUGLU

Samples:

sample_1: HEPES 20 mM; sodium chloride 100 mM; DTT 2 mM; K2F2 peptide 2 mM; ILK KLD/a-parvin CH2 50 uM; H2O 90%; D2O 10%

sample_conditions_1: temperature: 273 K; pH: 6.8; pressure: 1 atm; ionic strength: 100 mM

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1isotropicsample_conditions_1

Software:

XPLOR-NIH v2.25, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

PDB
DBJ BAE26194 BAE38850 BAF84427
EMBL CAA80852 CAD61925
GB AAH17327 AAH33436 AAH77819 AAH83876 AAI51293
REF NP_001011915 NP_001086955 NP_001094734 NP_001128471 NP_001128472
SP Q8CIB5 Q96AC1
TPG DAA25234