BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25214

Title: HIGH-RESOLUTION NMR STRUCTURES OF THE DOMAINS OF SACCHAROMYCES CEREVISIAE THO1

Deposition date: 2014-09-10 Original release date: 2014-12-15

Authors: Jacobsen, J.; Allen, Mark; Freund, S.; Bycroft, M.

Citation: Jacobsen, J.; Allen, Mark; Freund, S.; Bycroft, M.. "HIGH-RESOLUTION NMR STRUCTURES OF THE DOMAINS OF SACCHAROMYCES CEREVISIAE THO1"  Not known ., .-..

Assembly members:
PROTEIN_THO1, polymer, 67 residues, 7490.4826 Da.

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PROTEIN_THO1: GSALSPEEIKAKALDLLNKK LHRANKFGQDQADIDSLQRQ INRVEKFGVDLNSKLAEELG LVSRKNE

Data sets:
Data typeCount
1H chemical shifts496
13C chemical shifts225
15N chemical shifts66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PROTEIN THO11

Entities:

Entity 1, PROTEIN THO1 67 residues - 7490.4826 Da.

1   GLYSERALALEUSERPROGLUGLUILELYS
2   ALALYSALALEUASPLEULEUASNLYSLYS
3   LEUHISARGALAASNLYSPHEGLYGLNASP
4   GLNALAASPILEASPSERLEUGLNARGGLN
5   ILEASNARGVALGLULYSPHEGLYVALASP
6   LEUASNSERLYSLEUALAGLUGLULEUGLY
7   LEUVALSERARGLYSASNGLU

Samples:

sample_1: PROTEIN THO1, [U-13C; U-15N], 1.5 mM; potassium phosphate 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 120.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 293.000 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1solutionsample_conditions_1
1H-13C HSQCsample_1solutionsample_conditions_1
HNCACBsample_1solutionsample_conditions_1
CBCA(CO)NHsample_1solutionsample_conditions_1
HNCOsample_1solutionsample_conditions_1
HN(CA)COsample_1solutionsample_conditions_1
HNHBsample_1solutionsample_conditions_1
1H-NOESYsample_1solutionsample_conditions_1
1H-DQF-COSYsample_1solutionsample_conditions_1
1H-TOCSYsample_1solutionsample_conditions_1

Software:

Ansig vany, Kraulis - chemical shift assignment

AutoDep v4.3, PDBe - chemical shift assignment

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment, refinement

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

UNP THO1_YEAST
PDB
DBJ GAA22895
EMBL CAY79235
GB AAB64599 AAS56105 AHY75622 AJU40024 AJU40279
REF NP_010985
SP P40040
TPG DAA07722

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts