BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25217

Title: 1H, 13C, and 15N Chemical Shift Assignments for AUX/IAA17   PubMed: 25512488

Deposition date: 2014-09-11 Original release date: 2014-12-15

Authors: Suh, Jeong-Yong; Han, Mookyoung

Citation: Han, Mookyoung; Park, Yangshin; Kim, Iktae; Kim, Eun-Hee; Yu, Tae-Kyung; Rhee, Sangkee; Suh, Jeong-Yong. "Structural basis for the auxin-induced transcriptional regulation by Aux/IAA17"  Proc. Natl. Acad. Sci. U. S. A. 111, 18613-18618 (2014).

Assembly members:
Aux-IAA17, polymer, 113 residues, 12801.768 Da.

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Aux-IAA17: GGPEAAAFVKVSMDGAPYLR KIDLRMYKSYDELSNALSNM FSSFTMGKHGGEEGMIDFMN ERKLMDLVNSWDYVPSYENK DGNWMLVGDVPWPMFVDTCK RLRLMKGSDAIGL

Data sets:
Data typeCount
1H chemical shifts602
13C chemical shifts428
15N chemical shifts108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1AUX IAA171

Entities:

Entity 1, AUX IAA17 113 residues - 12801.768 Da.

1   GLYGLYPROGLUALAALAALAPHEVALLYS
2   VALSERMETASPGLYALAPROTYRLEUARG
3   LYSILEASPLEUARGMETTYRLYSSERTYR
4   ASPGLULEUSERASNALALEUSERASNMET
5   PHESERSERPHETHRMETGLYLYSHISGLY
6   GLYGLUGLUGLYMETILEASPPHEMETASN
7   GLUARGLYSLEUMETASPLEUVALASNSER
8   TRPASPTYRVALPROSERTYRGLUASNLYS
9   ASPGLYASNTRPMETLEUVALGLYASPVAL
10   PROTRPPROMETPHEVALASPTHRCYSLYS
11   ARGLEUARGLEUMETLYSGLYSERASPALA
12   ILEGLYLEU

Samples:

sample_1: Aux-IAA17, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 10 mM; DTT 10 mM; H2O 90%; D2O 10%

sample_conditions_1: temperature: 298 K; pH: 7.4; pressure: 1 atm; ionic strength: 0.01 M

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB AAB70451 AAB84354 AAC39439 AAC39440 AAG53997
REF NP_171921 XP_002889499
SP P93830

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts